5NRL

Structure of a pre-catalytic spliceosome.

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	2	U2 snRNA	polymer	1175	376267.4	1	GenBank (536627)	Saccharomyces cerevisiae
2	3	U6 snRNA-associated Sm-like protein LSm3	polymer	89	10039.3	1	UniProt (P57743) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (Baker's yeast)	SmX4 protein
3	4	U4 snRNA	polymer	160	51186.0	1	GenBank (1039024031)	Saccharomyces cerevisiae
4	5	U5 snRNA	polymer	214	68643.3	1	GenBank (1039024040)	Saccharomyces cerevisiae
5	6	U6 snRNA	polymer	112	35883.2	1	GenBank (1039023280)	Saccharomyces cerevisiae
6	7	U6 snRNA-associated Sm-like protein LSm7	polymer	115	13027.0	1	UniProt (P53905) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (Baker's yeast)
7	8	U6 snRNA-associated Sm-like protein LSm8	polymer	109	12403.4	1	UniProt (P47093) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (Baker's yeast)
8	A	Pre-mRNA-splicing factor 8	polymer	2413	279867.5	1	UniProt (P33334) Pfam (PF08084) Pfam (PF08082) Pfam (PF08083) Pfam (PF10598) Pfam (PF10597) Pfam (PF10596) Pfam (PF12134) In PDB	Saccharomyces cerevisiae (Baker's yeast)
9	B	Pre-mRNA-splicing helicase BRR2	polymer	2163	246470.3	1	UniProt (P32639) Pfam (PF21188) Pfam (PF18149) Pfam (PF00270) Pfam (PF00271) Pfam (PF02889) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Protein Snu246
10	C	Pre-mRNA-splicing factor SNU114	polymer	1008	114174.0	1	UniProt (P36048) Pfam (PF16004) Pfam (PF00009) Pfam (PF03764) Pfam (PF00679) In PDB	Saccharomyces cerevisiae (Baker's yeast)	114 kDa U5 small nuclear ribonucleoprotein component,Growth inhibitory protein 10
11	D	Spliceosomal protein DIB1	polymer	143	16798.4	1	UniProt (Q06819) Pfam (PF02966) In PDB	Saccharomyces cerevisiae (Baker's yeast)
12	E	66 kDa U4/U6.U5 small nuclear ribonucleoprotein component	polymer	587	66544.0	1	UniProt (Q12420) Pfam (PF19252) Pfam (PF03343) In PDB	Saccharomyces cerevisiae (Baker's yeast)
13	F	Pre-mRNA-processing factor 31	polymer	494	56382.5	1	UniProt (P49704) Pfam (PF01798) Pfam (PF09785) In PDB	Saccharomyces cerevisiae (Baker's yeast)
14	G	U4/U6 small nuclear ribonucleoprotein PRP3	polymer	469	55974.3	1	UniProt (Q03338) Pfam (PF08572) Pfam (PF06544) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Pre-mRNA-splicing factor 3
15	H	U4/U6 small nuclear ribonucleoprotein PRP4	polymer	465	52507.0	1	UniProt (P20053) Pfam (PF00400) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Pre-mRNA-processing protein 4
16	I	Yeast UBC4 gene for ubiquitin-conjugating enzyme	polymer	95	30200.7	1	GenBank (4718)	Saccharomyces cerevisiae
17	J	Pre-mRNA-splicing factor 6	polymer	899	104370.1	1	UniProt (P19735) Pfam (PF06424) In PDB	Saccharomyces cerevisiae (Baker's yeast)
18	K	13 kDa ribonucleoprotein-associated protein	polymer	126	13582.9	1	UniProt (P39990) Pfam (PF01248) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Small nuclear ribonucleoprotein-associated protein 1
19	L	23 kDa U4/U6.U5 small nuclear ribonucleoprotein component	polymer	194	22717.8	1	UniProt (Q12368) Pfam (PF12874) In PDB	Saccharomyces cerevisiae (Baker's yeast)
20	M	Pre-mRNA-splicing factor 38	polymer	242	27995.6	1	UniProt (Q00723) Pfam (PF03371) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Pre-mRNA-processing factor 38
21	N	Pre-mRNA-splicing factor SPP381	polymer	291	33830.9	1	UniProt (P38282) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Suppressor of PRP38-1 mutation
22	O	U2 snRNP component HSH155	polymer	971	110166.7	1	UniProt (P49955) Pfam (PF22646) In PDB	Saccharomyces cerevisiae (Baker's yeast)
23	P	Pre-mRNA-splicing factor RSE1	polymer	1361	153956.8	1	UniProt (Q04693) Pfam (PF10433) Pfam (PF03178) In PDB	Saccharomyces cerevisiae (Baker's yeast)	RNA splicing and ER to Golgi transport factor 1,Spliceosome-associated protein 130
24	Q	Cold sensitive U2 snRNA suppressor 1	polymer	435	50210.7	1	UniProt (Q02554) Pfam (PF04037) Pfam (PF04046) In PDB	Saccharomyces cerevisiae (Baker's yeast)
25	R	Protein HSH49	polymer	213	24534.2	1	UniProt (Q99181) Pfam (PF00076) In PDB	Saccharomyces cerevisiae (Baker's yeast)
26	S	Pre-mRNA-splicing factor RDS3	polymer	107	12283.6	1	UniProt (Q06835) Pfam (PF03660) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Regulator of drug sensitivity 3
27	T	Pre-mRNA-splicing factor PRP9	polymer	530	63126.4	1	UniProt (P19736) Pfam (PF16958) Pfam (PF16837) Pfam (PF12171) Pfam (PF11931) In PDB	Saccharomyces cerevisiae (Baker's yeast)
28	U	Pre-mRNA-splicing factor PRP11	polymer	266	29962.8	1	UniProt (Q07350) Pfam (PF12874) Pfam (PF16835) In PDB	Saccharomyces cerevisiae (Baker's yeast)
29	V	Pre-mRNA-splicing factor PRP21	polymer	280	33111.5	1	UniProt (P32524) Pfam (PF01805) Pfam (PF12230) In PDB	Saccharomyces cerevisiae (Baker's yeast)
30	W	U2 small nuclear ribonucleoprotein A'	polymer	238	27232.3	1	UniProt (Q08963) Pfam (PF14580) In PDB	Saccharomyces cerevisiae (Baker's yeast)	U2 snRNP A',Looks exceptionally like U2A protein 1
31	X	Unknown	polymer	111	7907.6	1		Saccharomyces cerevisiae
32	Y	U2 small nuclear ribonucleoprotein B''	polymer	111	12850.9	1	UniProt (P40567) Pfam (PF13893) In PDB	Saccharomyces cerevisiae (Baker's yeast)	U2 snRNP B''
33	Z	RDS3 complex subunit 10	polymer	85	10045.4	1	UniProt (P0C074) Pfam (PF07189) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Splicing factor 3b subunit
34	a	U6 snRNA-associated Sm-like protein LSm2	polymer	95	11177.9	1	UniProt (P38203) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Small nuclear ribonucleoprotein D homolog SNP3
35	b, k, s	Small nuclear ribonucleoprotein-associated protein B	polymer	196	22427.0	3	UniProt (P40018) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	snRNP-B,Sm protein B,SmB
36	d, n, v	Small nuclear ribonucleoprotein Sm D3	polymer	101	11240.1	3	UniProt (P43321) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Sm-D3,snRNP core protein D3
37	e, p, w	Small nuclear ribonucleoprotein E	polymer	94	10385.1	3	UniProt (Q12330) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	snRNP-E,Sm protein E,SmE
38	f, q, x	Small nuclear ribonucleoprotein F	polymer	86	9669.9	3	UniProt (P54999) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	snRNP-F,Sm protein F,SmF
39	g, r, y	Small nuclear ribonucleoprotein G	polymer	77	8490.8	3	UniProt (P40204) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	snRNP-G,Sm protein G,SmG
40	h, l, t	Small nuclear ribonucleoprotein Sm D1	polymer	146	16296.8	3	UniProt (Q02260) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Sm-D1,snRNP core protein D1
41	i, m, u	Small nuclear ribonucleoprotein Sm D2	polymer	110	12876.1	3	UniProt (Q06217) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Sm-D2,snRNP core protein D2
42	j	U6 snRNA-associated Sm-like protein LSm4	polymer	187	21298.1	1	UniProt (P40070) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (Baker's yeast)
43	o	U6 snRNA-associated Sm-like protein LSm5	polymer	93	10433.0	1	UniProt (P40089) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (Baker's yeast)
44	z	U6 snRNA-associated Sm-like protein LSm6	polymer	86	9406.6	1	UniProt (A6ZYX7) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (Baker's yeast)
45	C	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)
46	L, S, T, U	ZINC ION	non-polymer		65.4	7	Chemie (ZN)

Contents of the asymmetric unit

Polymers	Number of chains	58
	Total formula weight	2550727.6
Non-Polymers*	Number of molecules	8
	Total formula weight	981.0
All*	Total formula weight	2551708.6

*Water molecules are not included.