5NM3

Deinococcus radiodurans BphP PAS-GAF-PHY Y263F mutant, pre-illuminated

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Bacteriophytochrome	polymer	523	56565.0	4	UniProt (Q9RZA4) Pfam (PF08446) Pfam (PF01590) Pfam (PF00360)	Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)	Phytochrome-like protein
2	E, F, G, H (A, B, C, D)	3-[2-[(Z)-[3-(2-carboxyethyl)-5-[(Z)-(4-ethenyl-3-methyl-5-oxidanylidene-pyrrol-2-ylidene)methyl]-4-methyl-pyrrol-1-ium -2-ylidene]methyl]-5-[(Z)-[(3E)-3-ethylidene-4-methyl-5-oxidanylidene-pyrrolidin-2-ylidene]methyl]-4-methyl-1H-pyrrol-3- yl]propanoic acid	non-polymer		585.7	4	Chemie (LBV)
3	I, J, K (A, B, C)	water	water		18.0	5	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 502 (UniProt: Q9RZA4)

PDB	External Database	Details
Met -13	-	initiating methionine
Ala -12	-	expression tag
Ser -11	-	expression tag
Met -10	-	expression tag
Thr -9	-	expression tag
Gly -8	-	expression tag
Gly -7	-	expression tag
Gln -6	-	expression tag
Gln -5	-	expression tag
Met -4	-	expression tag
Gly -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Phe 263	Tyr 263	engineered mutation
Glu 503	-	expression tag
His 504	-	expression tag
His 505	-	expression tag
His 506	-	expression tag
His 507	-	expression tag
His 508	-	expression tag
His 509	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	226260.1
Non-Polymers*	Number of molecules	4
	Total formula weight	2342.7
All*	Total formula weight	228602.8

*Water molecules are not included.