5NL0

Crystal structure of a 197-bp palindromic 601L nucleosome in complex with linker histone H1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E, K (A, E, K)	Histone H3.2	polymer	135	15303.9	3	UniProt (P84233) Pfam (PF00125)	Xenopus laevis (African clawed frog)
2	B, F, L (B, F, L)	Histone H4	polymer	102	11263.2	3	UniProt (P62799) Pfam (PF15511)	Xenopus laevis (African clawed frog)
3	C, G, M (C, G, M)	Histone H2A type 1	polymer	129	13978.2	3	UniProt (P06897) Pfam (PF00125) Pfam (PF16211)	Xenopus laevis (African clawed frog)
4	D, H, N (D, H, N)	Histone H2B 1.1	polymer	122	13524.8	3	UniProt (P02281) Pfam (PF00125)	Xenopus laevis (African clawed frog)	H2B1.1
5	I, O (I, S)	DNA (197-MER)	polymer	197	60824.8	2		synthetic construct
6	J, P (J, T)	DNA (197-MER)	polymer	197	60815.8	2		synthetic construct
7	Q (Z)	Histone H1.0-B	polymer	196	21129.5	1	UniProt (P22844) Pfam (PF00538)	Xenopus laevis (African clawed frog)	H1-SA,H1D,Histone H1(0)-2,Histone H5A,XlH5A

Sequence modifications

A, E, K: 1 - 135 (UniProt: P84233)

PDB	External Database	Details
Ala 102	Gly 103	engineered mutation

C, G, M: 1 - 129 (UniProt: P06897)

PDB	External Database	Details
Arg 99	Gly 100	conflict
Ser 123	Ala 124	conflict

D, H, N: 1 - 122 (UniProt: P02281)

PDB	External Database	Details
Thr 29	Ser 33	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	17
	Total formula weight	426621.0
All*	Total formula weight	426621.0

*Water molecules are not included.