5NL0
Crystal structure of a 197-bp palindromic 601L nucleosome in complex with linker histone H1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-08-29 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.99987 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 61.725, 405.737, 348.162 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.128 - 5.400 |
R-factor | 0.2422 |
Rwork | 0.240 |
R-free | 0.26530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ut9 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.334 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.130 | 6.040 |
High resolution limit [Å] | 5.400 | 5.400 |
Rmerge | 0.091 | 0.685 |
Rpim | 0.072 | 0.553 |
Number of reflections | 15641 | 4370 |
<I/σ(I)> | 9.1 | 2.1 |
Completeness [%] | 99.5 | 99.6 |
Redundancy | 4.5 | 4.4 |
CC(1/2) | 0.997 | 0.686 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293.15 | Mix of equal volumes of the nucleosome/H1 complex (25-30 microM) and a crystallization solution composed of MPD (6% v/v), 50 mM NaCl, and 50 mM sodium potassium phosphate pH 6.4. |