5NHN

Super-Folder Green Fluorescent Protein Artificiall dimer linked via 148 position

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B	Green fluorescent protein	polymer	230	25821.1	1	UniProt (A0A059PIQ0) Pfam (PF01353) UniProt (by SIFTS) (P42212) In PDB	Aequorea victoria (Jellyfish)
2	A	Green fluorescent protein	polymer	230	25839.1	1	UniProt (A0A059PIQ0) Pfam (PF01353) UniProt (by SIFTS) (P42212) In PDB	Aequorea victoria (Jellyfish)
3	B	[(4~{S})-4,5,6,7,8,9-hexahydro-1~{H}-cycloocta[d][1,2,3]triazol-4-yl] hydrogen carbonate	non-polymer		211.2	1	Chemie (DB5)
4	B	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
5	B	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
6		water	water		18.0	245	Chemie (HOH)

Sequence modifications

B: 3 - 231 (UniProt: A0A059PIQ0)

PDB	External Database	Details
Ser 2	-	expression tag
Arg 30	Ser 30	engineered mutation
Cro 66	Thr 65	chromophore
Cro 66	Tyr 66	chromophore
Cro 66	Gly 67	chromophore
Ser 72	Ala 72	engineered mutation
Arg 80	Gln 80	engineered mutation
Lys 148	His 148	engineered mutation
Val 206	Ala 206	engineered mutation

A: 3 - 231 (UniProt: A0A059PIQ0)

PDB	External Database	Details
Ser 2	-	expression tag
Arg 30	Ser 30	engineered mutation
Cro 66	Thr 65	chromophore
Cro 66	Tyr 66	chromophore
Cro 66	Gly 67	chromophore
Ser 72	Ala 72	engineered mutation
Arg 80	Gln 80	engineered mutation
Phe 148	His 148	engineered mutation
Val 206	Ala 206	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	51660.1
Non-Polymers*	Number of molecules	3
	Total formula weight	365.4
All*	Total formula weight	52025.5

*Water molecules are not included.