5NHN
Super-Folder Green Fluorescent Protein Artificiall dimer linked via 148 position
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-18 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 65 |
| Unit cell lengths | 99.800, 99.800, 108.920 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 67.710 - 1.960 |
| R-factor | 0.18104 |
| Rwork | 0.180 |
| R-free | 0.20895 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5bto |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.868 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 67.710 |
| High resolution limit [Å] | 1.960 |
| Rmerge | 0.079 |
| Number of reflections | 41916 |
| <I/σ(I)> | 22.9 |
| Completeness [%] | 100.0 |
| Redundancy | 21.8 |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 300 | Condition C6 of the PACT Premier screen: 0.1 M PCTP Buffer, pH 9.0, 25% PEG 1500 |
