5NG1

TUBULIN-MTC-zampanolide complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Tubulin alpha-1B chain	polymer	451	50204.4	2	UniProt (P81947) Pfam (PF00091) Pfam (PF03953) In PDB	Bos taurus (cattle)	Alpha-tubulin ubiquitous,Tubulin K-alpha-1,Tubulin alpha-ubiquitous chain
2	B, D	Tubulin beta-2B chain	polymer	445	49999.9	2	UniProt (Q6B856) Pfam (PF00091) Pfam (PF03953) In PDB	Bos taurus (cattle)
3	E	Stathmin-4	polymer	143	16844.2	1	UniProt (P63043) Pfam (PF00836) In PDB	Rattus norvegicus (Norway Rat)	Stathmin-like protein B3,RB3
4	F	Tubulin-Tyrosine Ligase	polymer	384	44378.5	1	UniProt (E1BQ43) In PDB	Gallus gallus (Chicken)
5	A, C	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)
6	A, B, C, D, F	MAGNESIUM ION	non-polymer		24.3	5	Chemie (MG)
7	A, C	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
8	B, D	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP)
9	B, D	2-methoxy-5-(2,3,4-trimethoxyphenyl)cyclohepta-2,4,6-trien-1-one	non-polymer		302.3	2	Chemie (8WB)
10	B	(2~{Z},4~{E})-~{N}-[(~{S})-oxidanyl-[(1~{S},2~{E},5~{S},11~{R},17~{S},19~{R})-3,11,19-trimethyl-7,13-bis(oxidanylidene)-6,21-dioxabicyclo[15.3.1]henicos-2-en-5-yl]methyl]hexa-2,4-dienamide	non-polymer		503.7	1	Chemie (8WE)
11	B	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
12	B	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)
13	D	(2Z,4E)-N-[(S)-[(1S,2E,5S,8E,10Z,17S)-3,11-dimethyl-19-methylidene-7,13-dioxo-6,21-dioxabicyclo[15.3.1]henicosa-2,8,10-trien-5-yl](hydroxy)methyl]hexa-2,4-dienamide	non-polymer		497.6	1	Chemie (ZPN)
14	F	PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER	non-polymer		505.2	1	Chemie (ACP)
15		water	water		18.0	570	Chemie (HOH)

Sequence modifications

E: 5 - 145 (UniProt: P63043)

PDB	External Database	Details
Met 3	-	initiating methionine
Ala 4	-	expression tag

F: 1 - 378 (UniProt: E1BQ43)

PDB	External Database	Details
His 379	-	expression tag
His 380	-	expression tag
His 381	-	expression tag
His 382	-	expression tag
His 383	-	expression tag
His 384	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	261631.3
Non-Polymers*	Number of molecules	18
	Total formula weight	4532.9
All*	Total formula weight	266164.2

*Water molecules are not included.