5NFX

Deinococcus radiodurans BphP PAS-GAF Y263F mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Bacteriophytochrome	polymer	343	37213.5	1	UniProt (Q9RZA4) Pfam (PF08446) Pfam (PF01590)	Deinococcus radiodurans R1	Phytochrome-like protein
2	B, C, D (A)	ACETATE ION	non-polymer		59.0	3	Chemie (ACT)
3	E (A)	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	1	Chemie (MPD)
4	F (A)	3-[2-[(Z)-[3-(2-carboxyethyl)-5-[(Z)-(4-ethenyl-3-methyl-5-oxidanylidene-pyrrol-2-ylidene)methyl]-4-methyl-pyrrol-1-ium -2-ylidene]methyl]-5-[(Z)-[(3E)-3-ethylidene-4-methyl-5-oxidanylidene-pyrrolidin-2-ylidene]methyl]-4-methyl-1H-pyrrol-3- yl]propanoic acid	non-polymer		585.7	1	Chemie (LBV)
5	G (A)	water	water		18.0	318	Chemie (HOH)

Sequence modifications

A: 1 - 321 (UniProt: Q9RZA4)

PDB	External Database	Details
Met -13	-	initiating methionine
Ala -12	-	expression tag
Ser -11	-	expression tag
Met -10	-	expression tag
Thr -9	-	expression tag
Gly -8	-	expression tag
Gly -7	-	expression tag
Gln -6	-	expression tag
Gln -5	-	expression tag
Met -4	-	expression tag
Gly -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Phe 263	Tyr 263	engineered mutation
Leu 322	-	expression tag
Glu 323	-	expression tag
His 324	-	expression tag
His 325	-	expression tag
His 326	-	expression tag
His 327	-	expression tag
His 328	-	expression tag
His 329	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	37213.5
Non-Polymers*	Number of molecules	5
	Total formula weight	881.0
All*	Total formula weight	38094.4

*Water molecules are not included.