5NBN

Crystal structure of the Arp4-N-actin-Arp8-Ino80HSA module of INO80

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Actin-related protein 4	polymer	489	54894.7	2	UniProt (P80428) Pfam (PF00022)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Actin-like protein ARP4,Actin-like protein 4
2	C, D (C, D)	Actin	polymer	375	41748.6	2	UniProt (P60010) Pfam (PF00022)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
3	E, F (E, F)	Actin-like protein ARP8	polymer	628	71987.5	2	UniProt (Q12386) Pfam (PF00022)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
4	G, H (G, H)	Putative DNA helicase INO80	polymer	147	18020.7	2	UniProt (P53115) Pfam (PF13892)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Inositol-requiring protein 80
5	I, K, M, P (A, B, C, D)	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
6	J, L, N, Q (A, B, C, D)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	4	Chemie (ATP)
7	O, R (C, D)	LATRUNCULIN A	non-polymer		421.6	2	Chemie (LAR)

Sequence modifications

E, F: 255 - 881 (UniProt: Q12386)

PDB	External Database	Details
Met 254	-	initiating methionine

G, H: 462 - 598 (UniProt: P53115)

PDB	External Database	Details
Met 461	-	initiating methionine
Asn 599	-	expression tag
Trp 600	-	expression tag
Ser 601	-	expression tag
His 602	-	expression tag
Pro 603	-	expression tag
Gln 604	-	expression tag
Phe 605	-	expression tag
Glu 606	-	expression tag
Lys 607	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	373302.8
Non-Polymers*	Number of molecules	10
	Total formula weight	3032.1
All*	Total formula weight	376334.9

*Water molecules are not included.