5NBN

Crystal structure of the Arp4-N-actin-Arp8-Ino80HSA module of INO80

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	C 2 2 21
Unit cell lengths	172.294, 263.909, 241.402
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.399 - 4.000
R-factor	0.1953
Rwork	0.193
R-free	0.24220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4am6
RMSD bond length	0.002
RMSD bond angle	0.675
Data reduction software	XDS
Data scaling software	Aimless (0.5.27)
Phasing software	PHASER (2.6.1)
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.430	49.430	4.140
High resolution limit [Å]	4.000	15.490	4.000
Rmerge	0.289	0.071	1.345
Rmeas	0.306	0.075	1.418
Rpim	0.098	0.025	0.445
Number of reflections	46724
<I/σ(I)>	8.2	26.4	2.3
Completeness [%]	99.9	96.8	100
Redundancy	9.6	8.4	10
CC(1/2)	0.994	0.996	0.688

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M sodium citrate tribasic dihydrate, 18% (w/v) polyethylene glycol 3,350, latrunculin A solved in DMSO molar ration 1:1.5 (protein : latrunculin A)