5N6N

CRYSTAL STRUCTURE OF THE 14-3-3:NEUTRAL TREHALASE NTH1 COMPLEX

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Protein BMH1	polymer	240	27372.3	2	UniProt (P29311) Pfam (PF00244) In PDB	Saccharomyces cerevisiae S288c
2	C	Neutral trehalase	polymer	756	86670.5	1	UniProt (P32356) Pfam (PF07492) Pfam (PF01204) In PDB	Saccharomyces cerevisiae S288c	Alpha,alpha-trehalase,alpha-trehalose glucohydrolase
3	D, E, F	beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose	branched		342.3	3	In PDB BIRD (PRD_900003)		sucrose
4	C	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
5		water	water		18.0	448	Chemie (HOH)

Sequence modifications

A, B: 1 - 236 (UniProt: P29311)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag

C: 1 - 751 (UniProt: P32356)

PDB	External Database	Details
Gly -4	-	expression tag
Ser -3	-	expression tag
Ala -2	-	expression tag
Met -1	-	expression tag
Ala 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	141415.1
Branched	Number of molecules	3
	Total formula weight	1026.9
Non-Polymers*	Number of molecules	1
	Total formula weight	40.1
All*	Total formula weight	142482.1

*Water molecules are not included.