5N6N
CRYSTAL STRUCTURE OF THE 14-3-3:NEUTRAL TREHALASE NTH1 COMPLEX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 160.805, 104.749, 105.638 |
Unit cell angles | 90.00, 121.89, 90.00 |
Refinement procedure
Resolution | 46.155 - 2.290 |
R-factor | 0.1861 |
Rwork | 0.184 |
R-free | 0.23740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5jta homology model of Bmh1 based on 2BR9 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.942 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.710 | 2.420 |
High resolution limit [Å] | 2.290 | 2.290 |
Number of reflections | 65450 | 5711 |
<I/σ(I)> | 11.79 | 1.78 |
Completeness [%] | 97.2 | 85.5 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 291.15 | 100 mM sodium cacodylate, 200 mM calcium acetate, 18% (w/v) PEG 8000, 12% (w/v) sucrose |