5N4W

Crystal structure of the Cul2-Rbx1-EloBC-VHL ubiquitin ligase complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Cullin-2	polymer	748	87300.1	1	UniProt (Q13617) Pfam (PF00888) Pfam (PF10557) In PDB	Homo sapiens (Human)	CUL-2
2	V	Von Hippel-Lindau disease tumor suppressor	polymer	160	18558.2	1	UniProt (P40337) Pfam (PF01847) Pfam (PF17211) In PDB	Homo sapiens (Human)	Protein G7,pVHL
3	R	E3 ubiquitin-protein ligase RBX1	polymer	102	11599.2	1	UniProt (P62877) Pfam (PF12678) In PDB	Homo sapiens (Human)	Protein ZYP,RING finger protein 75,RING-box protein 1,Rbx1,Regulator of cullins 1
4	B	Elongin-B	polymer	104	11748.4	1	UniProt (Q15370) Pfam (PF00240) In PDB	Homo sapiens (Human)	EloB,Elongin 18 kDa subunit,RNA polymerase II transcription factor SIII subunit B,SIII p18,Transcription elongation factor B polypeptide 2
5	C	Elongin-C	polymer	97	10974.6	1	UniProt (Q15369) Pfam (PF03931) In PDB	Homo sapiens (Human)	EloC,Elongin 15 kDa subunit,RNA polymerase II transcription factor SIII subunit C,SIII p15,Transcription elongation factor B polypeptide 1
6	R	ZINC ION	non-polymer		65.4	3	Chemie (ZN)	Homo sapiens (Human)

Sequence modifications

A: 1 - 745 (UniProt: Q13617)

PDB	External Database	Details
Gly -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

R: 1 - 102 (UniProt: P62877)

PDB	External Database	Details
Lys 18	Gly 18	conflict

C: 17 - 112 (UniProt: Q15369)

PDB	External Database	Details
Met 16	-	initiating methionine

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	140180.5
Non-Polymers*	Number of molecules	3
	Total formula weight	196.2
All*	Total formula weight	140376.8

*Water molecules are not included.