5N47

Structure of Anticalin N7E in complex with the three-domain fragment Fn7B8 of human oncofetal fibronectin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E	Neutrophil gelatinase-associated lipocalin	polymer	188	21727.7	3	UniProt (P80188) Pfam (PF00061) In PDB	Homo sapiens (Human)	NGAL,25 kDa alpha-2-microglobulin-related subunit of MMP-9,Lipocalin-2,Oncogene 24p3,Siderocalin LCN2,p25
2	B, D, F	Fibronectin	polymer	284	30834.9	3	UniProt (P02751) Pfam (PF00041) In PDB	Homo sapiens (Human)	FN,Cold-insoluble globulin,CIG

Sequence modifications

A, C, E: 1 - 178 (UniProt: P80188)

PDB	External Database	Details
His 28	Gln 48	engineered mutation
Glu 36	Leu 56	engineered mutation
Ser 40	Ala 60	engineered mutation
Leu 41	Ile 61	engineered mutation
Arg 49	Gln 69	engineered mutation
Arg 70	Leu 90	engineered mutation
Ser 73	Lys 93	engineered mutation
His 77	Asp 97	engineered mutation
Leu 79	Trp 99	engineered mutation
Ser 87	Cys 107	engineered mutation
Leu 96	Asn 116	engineered mutation
Lys 100	Tyr 120	engineered mutation
His 103	Leu 123	engineered mutation
Phe 106	Tyr 126	engineered mutation
Thr 125	Lys 145	engineered mutation
Ala 127	Ser 147	engineered mutation
Phe 134	Lys 154	engineered mutation
Ser 179	-	expression tag
Ala 180	-	expression tag
Trp 181	-	expression tag
Ser 182	-	expression tag
His 183	-	expression tag
Pro 184	-	expression tag
Gln 185	-	expression tag
Phe 186	-	expression tag
Glu 187	-	expression tag
Lys 188	-	expression tag

B, D, F: 1173 - 1448 (UniProt: P02751)

PDB	External Database	Details
Ser 1449	-	expression tag
Ala 1450	-	expression tag
His 1451	-	expression tag
His 1452	-	expression tag
His 1453	-	expression tag
His 1454	-	expression tag
His 1455	-	expression tag
His 1456	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	157687.8
All*	Total formula weight	157687.8

*Water molecules are not included.