5MU3

Crystal structure of Ctf19-Mcm21 kinetochore assembly bound with Ctf19-Mcm21 binding motif of central kinetochore subunit Okp1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D	Central kinetochore subunit MCM21	polymer	189	22071.3	2	UniProt (Q6CVQ9) Pfam (PF09496) In PDB	Kluyveromyces lactis NRRL Y-1140	Minichromosome maintenance protein 21
2	B	Central kinetochore subunit CTF19	polymer	165	19046.1	1	UniProt (Q6CRN7) In PDB	Kluyveromyces lactis NRRL Y-1140	Chromosome transmission fidelity protein 19
3	C, F	Central kinetochore subunit Okp1	polymer	67	7616.6	2	UniProt (Q6CJY0) In PDB	Kluyveromyces lactis NRRL Y-1140	Okp1
4	E	Central kinetochore subunit CTF19	polymer	165	19062.1	1	UniProt (Q6CRN7) In PDB	Kluyveromyces lactis NRRL Y-1140	Chromosome transmission fidelity protein 19
5		water	water		18.0	338	Chemie (HOH)

Sequence modifications

A, D: 108 - 293 (UniProt: Q6CVQ9)

PDB	External Database	Details
Ser -3	-	expression tag
Asn -2	-	expression tag
Ala -1	-	expression tag

B: 107 - 270 (UniProt: Q6CRN7)

PDB	External Database	Details
Met 106	-	initiating methionine

C, F: 295 - 360 (UniProt: Q6CJY0)

PDB	External Database	Details
Met 294	-	initiating methionine

E: 107 - 270 (UniProt: Q6CRN7)

PDB	External Database	Details
Met 106	-	initiating methionine

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	97484.0
All*	Total formula weight	97484.0

*Water molecules are not included.