5MU3

Crystal structure of Ctf19-Mcm21 kinetochore assembly bound with Ctf19-Mcm21 binding motif of central kinetochore subunit Okp1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 2 21 21
Unit cell lengths	93.342, 105.476, 122.982
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	80.063 - 2.100
R-factor	0.2223
Rwork	0.222
R-free	0.24830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3zxu
RMSD bond length	0.002
RMSD bond angle	0.456
Data reduction software	XDS (January 2014)
Data scaling software	Aimless (0.1.27)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	122.980	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.098	3.446
Rpim	0.073	2.533
Number of reflections	71441	4549
<I/σ(I)>	10.1	0.5
Completeness [%]	99.9	99.9
Redundancy	5.3	5.4
CC(1/2)	0.998	0.192

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	35 % (v/v) glycerol ethoxylate 200 mM Li-citrate