5MSG

Influenza B polymerase bound to vRNA promoter and capped RNA primer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Polymerase acidic protein	polymer	751	85822.8	1	UniProt (Q5V8Z9) Pfam (PF00603)	Influenza B virus
2	B (B)	RNA-directed RNA polymerase catalytic subunit	polymer	772	86207.0	1	UniProt (Q5V8Y6) Pfam (PF00602)	Influenza B virus
3	C (C)	Polymerase basic protein 2	polymer	798	90844.1	1	UniProt (Q5V8X3) Pfam (PF20947) Pfam (PF20948) Pfam (PF20949) Pfam (PF20950) Pfam (PF00604) Pfam (PF20951) Pfam (PF20952)	Influenza B virus
4	D (M)	RNA (5'-D(*(GDM))-R(P*AP*AP*UP*C)-3')	polymer	14	4550.7	1		synthetic construct
5	E (R)	RNA (5'-R(*UP*AP*UP*AP*CP*CP*UP*CP*UP*GP*CP*UP*UP*CP*UP*GP*CP*U)-3')	polymer	18	5584.3	1		Influenza B virus
6	F (V)	RNA (5'-R(P*AP*GP*UP*AP*GP*UP*AP*AP*CP*AP*AP*GP*AP*G)-3')	polymer	14	4557.8	1		Influenza B virus
7	G, H (A, B)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)

Sequence modifications

A: 1 - 726 (UniProt: Q5V8Z9)

PDB	External Database	Details
Gly -13	-	expression tag
Ser -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Gly 727	-	expression tag
Ser 728	-	expression tag
Gly 729	-	expression tag
Ser 730	-	expression tag
Gly 731	-	expression tag
Glu 732	-	expression tag
Asn 733	-	expression tag
Leu 734	-	expression tag
Tyr 735	-	expression tag
Phe 736	-	expression tag
Gln 737	-	expression tag

B: 1 - 752 (UniProt: Q5V8Y6)

PDB	External Database	Details
Gly -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Ser -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Gly 753	-	expression tag
Ser 754	-	expression tag
Gly 755	-	expression tag
Ser 756	-	expression tag
Gly 757	-	expression tag
Glu 758	-	expression tag
Asn 759	-	expression tag
Leu 760	-	expression tag
Tyr 761	-	expression tag
Phe 762	-	expression tag
Gln 763	-	expression tag

C: 1 - 770 (UniProt: Q5V8X3)

PDB	External Database	Details
Gly -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Ser -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Gly 771	-	expression tag
Trp 772	-	expression tag
Ser 773	-	expression tag
His 774	-	expression tag
Pro 775	-	expression tag
Gln 776	-	expression tag
Phe 777	-	expression tag
Glu 778	-	expression tag
Lys 779	-	expression tag
Gly 780	-	expression tag
Ser 781	-	expression tag
Gly 782	-	expression tag
Ser 783	-	expression tag
Glu 784	-	expression tag
Asn 785	-	expression tag
Leu 786	-	expression tag
Tyr 787	-	expression tag
Phe 788	-	expression tag
Gln 789	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	277566.7
Non-Polymers*	Number of molecules	2
	Total formula weight	189.9
All*	Total formula weight	277756.7

*Water molecules are not included.