5MR3

Crystal structure of red abalone egg VERL repeat 2 with linker in complex with sperm lysin at 1.8 A resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G (A, C, E, G)	Egg-lysin	polymer	136	16295.2	4	UniProt (P04552) Pfam (PF01303)	Haliotis rufescens (California red abalone)	Sperm-lysin
2	B, D, F, H (B, D, F, H)	Vitelline envelope sperm lysin receptor	polymer	134	15089.3	4	UniProt (Q8WR62) Pfam (PF11386)	Haliotis rufescens (California red abalone)
3	AA, I, J, K, M... (H, A, B, C, D...)	GLYCEROL	non-polymer		92.1	12	Chemie (GOL)
4	L, V (B, F)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
5	BA, N, S, W (H, B, D, F)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)	Haliotis rufescens
6	X, Y (G)	TRIETHYLENE GLYCOL	non-polymer		150.2	2	Chemie (PGE)
7	CA, DA, EA, FA, GA... (A, B, C, D, E...)	water	water		18.0	559	Chemie (HOH)

Sequence modifications

B, D, F, H: 176 - 298 (UniProt: Q8WR62)

PDB	External Database	Details
Glu 173	-	expression tag
Thr 174	-	expression tag
Gly 175	-	expression tag
Ala 293	Ser 293	engineered mutation
Ala 296	Ser 296	engineered mutation
Ala 297	Ser 297	engineered mutation
Leu 299	-	expression tag
Glu 300	-	expression tag
His 301	-	expression tag
His 302	-	expression tag
His 303	-	expression tag
His 304	-	expression tag
His 305	-	expression tag
His 306	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	125538.2
Non-Polymers*	Number of molecules	20
	Total formula weight	2361.2
All*	Total formula weight	127899.4

*Water molecules are not included.