5MQ5
A protease-resistant N24S Escherichia coli Asparaginase mutant with outstanding stability and enhanced anti-leukaemic activity
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, C, B, D) | L-asparaginase 2 | polymer | 332 | 35428.7 | 4 | UniProt (P00805) Pfam (PF00710) Pfam (PF17763) | Escherichia coli | L-asparaginase II,L-ASNase II,L-asparagine amidohydrolase II |
| 2 | E, F, G, H (A, C, B, D) | ASPARTIC ACID | non-polymer | 133.1 | 4 | Chemie (ASP) | |||
| 3 | I, J, K, L (A, C, B, D) | water | water | 18.0 | 1225 | Chemie (HOH) |
Sequence modifications
A, C, B, D: 1 - 326 (UniProt: P00805)
| PDB | External Database | Details |
|---|---|---|
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| His -1 | - | expression tag |
| His 0 | - | expression tag |
| Ser 24 | Asn 46 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 141714.6 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 532.4 | |
| All* | Total formula weight | 142247.1 |
