5MQ5

A protease-resistant N24S Escherichia coli Asparaginase mutant with outstanding stability and enhanced anti-leukaemic activity

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, B, D	L-asparaginase 2	polymer	332	35428.7	4	UniProt (P00805) Pfam (PF00710) Pfam (PF17763) In PDB	Escherichia coli	L-asparaginase II,L-ASNase II,L-asparagine amidohydrolase II
2	A, C, B, D	ASPARTIC ACID	non-polymer		133.1	4	Chemie (ASP)
3		water	water		18.0	1225	Chemie (HOH)

Sequence modifications

A, C, B, D: 1 - 326 (UniProt: P00805)

PDB	External Database	Details
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Ser 24	Asn 46	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	141714.6
Non-Polymers*	Number of molecules	4
	Total formula weight	532.4
All*	Total formula weight	142247.1

*Water molecules are not included.