5MPV

Crystal structure of a Mycobacterium tuberculosis chorismate mutase optimized for high autonomous activity by directed evolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	D	Intracellular chorismate mutase	polymer	90	10063.8	1	UniProt (P9WIC1) Pfam (PF01817) In PDB	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	CM
2		water	water		18.0	71	Chemie (HOH)

Sequence modifications

D: 1 - 78 (UniProt: P9WIC1)

PDB	External Database	Details
Leu 11	Val 26	engineered mutation
Val 15	Asp 30	engineered mutation
Gln 40	Lys 55	engineered mutation
Pro 52	Thr 67	engineered mutation
Asp 55	Val 70	engineered mutation
Ile 62	Val 77	engineered mutation
Val 72	Asp 87	engineered mutation
Leu 79	-	expression tag
Leu 80	-	expression tag
Leu 81	-	expression tag
Arg 82	-	expression tag
Leu 83	-	expression tag
Gly 84	-	expression tag
Arg 85	-	expression tag
Gly 86	-	expression tag
Pro 87	-	expression tag
Asp 88	-	expression tag
Ala 89	-	expression tag
Met 90	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	10063.8
All*	Total formula weight	10063.8

*Water molecules are not included.