5MPS

Structure of a spliceosome remodeled for exon ligation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	I	Yeast UBC4 gene for ubiquitin-conjugating enzyme	polymer	95	30200.7	1	GenBank (4718)	Saccharomyces cerevisiae (Baker's yeast)
2	E	UBC4 gene exon	polymer	20	6519.0	1		Saccharomyces cerevisiae (Baker's yeast)
3	2	U2 snRNA	polymer	1175	376267.4	1	GenBank (536627)	Saccharomyces cerevisiae (Baker's yeast)
4	6	Saccharomyces cerevisiae strain T.52_2H chromosome XII sequence	polymer	112	35883.2	1	GenBank (1039022925)	Saccharomyces cerevisiae (Baker's yeast)
5	5	U5 snRNA	polymer	179	57444.9	1	GenBank (1039023700)	Saccharomyces cerevisiae (Baker's yeast)
6	A	Pre-mRNA-splicing factor 8	polymer	2413	279867.5	1	UniProt (P33334) Pfam (PF08082) Pfam (PF08083) Pfam (PF10598) Pfam (PF10597) Pfam (PF10596) Pfam (PF12134) Pfam (PF08084) In PDB	Saccharomyces cerevisiae (Baker's yeast)
7	C	Pre-mRNA-splicing factor SNU114	polymer	1008	114174.0	1	UniProt (P36048) Pfam (PF03764) Pfam (PF00679) Pfam (PF16004) Pfam (PF00009) In PDB	Saccharomyces cerevisiae (Baker's yeast)	114 kDa U5 small nuclear ribonucleoprotein component,Growth inhibitory protein 10
8	H	Pre-mRNA-splicing factor CWC22	polymer	577	67386.1	1	UniProt (P53333) Pfam (PF02847) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Complexed with CEF1 protein 22
9	J	Pre-mRNA-splicing factor PRP46	polymer	451	50771.3	1	UniProt (Q12417) Pfam (PF00400) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Complexed with CEF1 protein 1,PRP nineteen-associated complex protein 50,PRP19-associated complex protein 50,Pre-mRNA-processing protein 46
10	K	Pre-mRNA-processing protein 45	polymer	379	42548.7	1	UniProt (P28004) Pfam (PF02731) In PDB	Saccharomyces cerevisiae (Baker's yeast)
11	L	Pre-mRNA-splicing factor BUD31	polymer	157	18484.5	1	UniProt (P25337) Pfam (PF01125) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Bud site selection protein 31,Complexed with CEF1 protein 14
12	M	Pre-mRNA-splicing factor CWC2	polymer	339	38486.6	1	UniProt (Q12046) Pfam (PF16131) Pfam (PF00076) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Complexed with CEF1 protein 2,PRP19-associated complex protein 40,Synthetic lethal with CLF1 protein 3
13	N	Pre-mRNA-splicing factor SLT11	polymer	364	40988.6	1	UniProt (P38241) Pfam (PF21369) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Extracellular mutant protein 2,Synthetic lethality with U2 protein 11
14	O	Pre-mRNA-splicing factor CEF1	polymer	590	67837.8	1	UniProt (Q03654) Pfam (PF00249) Pfam (PF11831) In PDB	Saccharomyces cerevisiae (Baker's yeast)	PRP nineteen-associated complex protein 85,PRP19-associated complex protein 85
15	P	Pre-mRNA-splicing factor CWC15	polymer	175	19975.2	1	UniProt (Q03772) Pfam (PF04889) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Complexed with CEF1 protein 15
16	R	Pre-mRNA-splicing factor CWC21	polymer	135	15793.6	1	UniProt (Q03375) Pfam (PF08312) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Complexed with CEF1 protein 21
17	S	Pre-mRNA-splicing factor CLF1	polymer	687	82555.9	1	UniProt (Q12309) Pfam (PF05843) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Crooked neck-like factor 1,PRP19-associated complex protein 77,Synthetic lethal with CDC40 protein 3
18	T	Pre-mRNA-splicing factor SYF1	polymer	877	101875.9	1	UniProt (Q04048) Pfam (PF05843) In PDB	Saccharomyces cerevisiae (Baker's yeast)	PRP19-associated complex protein 90,Synthetic lethal with CDC40 protein 1
19	a	Pre-mRNA-splicing factor 18	polymer	251	28414.4	1	UniProt (P33411) Pfam (PF02840) In PDB	Saccharomyces cerevisiae (Baker's yeast)
20	c	Pre-mRNA-splicing factor SLU7	polymer	382	44722.9	1	UniProt (Q02775) Pfam (PF11708) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Synthetic lethal with U2 snRNA protein 17,Synthetic lethal with U5 snRNA protein 7
21	o	Pre-mRNA-processing factor 17	polymer	455	52128.8	1	UniProt (P40968) Pfam (PF00400) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Cell division control protein 40
22	X	Unknown	polymer	68	5805.1	1		Saccharomyces cerevisiae (Baker's yeast)
23	y	Pre-mRNA-splicing factor SYF2	polymer	215	24850.7	1	UniProt (P53277) Pfam (PF08231) In PDB	Saccharomyces cerevisiae (Baker's yeast)	PRP19 complex protein 31,Synthetic lethal with CDC40 protein 2
24	b	Small nuclear ribonucleoprotein-associated protein B	polymer	196	22427.0	1	UniProt (P40018) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (Baker's yeast)	snRNP-B,Sm protein B,SmB
25	d	Small nuclear ribonucleoprotein Sm D3	polymer	101	11240.1	1	UniProt (P43321) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Sm-D3,snRNP core protein D3
26	e	Small nuclear ribonucleoprotein E	polymer	94	10385.1	1	UniProt (Q12330) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (Baker's yeast)	snRNP-E,Sm protein E,SmE
27	f	Small nuclear ribonucleoprotein F	polymer	86	9669.9	1	UniProt (P54999) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (Baker's yeast)	snRNP-F,Sm protein F,SmF
28	g	Small nuclear ribonucleoprotein G	polymer	77	8490.8	1	UniProt (P40204) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (Baker's yeast)	snRNP-G,Sm protein G,SmG
29	h	Small nuclear ribonucleoprotein Sm D1	polymer	146	16296.8	1	UniProt (Q02260) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Sm-D1,snRNP core protein D1
30	j	Small nuclear ribonucleoprotein Sm D2	polymer	110	12876.1	1	UniProt (Q06217) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Sm-D2,snRNP core protein D2
31	6	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
32	6	POTASSIUM ION	non-polymer		39.1	2	Chemie (K)
33	A	INOSITOL HEXAKISPHOSPHATE	non-polymer		660.0	1	Chemie (IHP)
34	C	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)
35	L, M, N	ZINC ION	non-polymer		65.4	6	Chemie (ZN)

Sequence modifications

T: 1 - 859 (UniProt: Q04048)
T: 1000 - 1017 (PDB: 5MPS)

Contents of the asymmetric unit

Polymers	Number of chains	30
	Total formula weight	1694368.4
Non-Polymers*	Number of molecules	13
	Total formula weight	1726.8
All*	Total formula weight	1696095.2

*Water molecules are not included.