5MIO
KIF2C-DARPIN FUSION PROTEIN BOUND TO TUBULIN
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Tubulin alpha-1B chain | polymer | 451 | 50204.4 | 1 | UniProt (P81947) Pfam (PF00091) Pfam (PF03953) | Bos taurus (cattle) | Alpha-tubulin ubiquitous,Tubulin K-alpha-1,Tubulin alpha-ubiquitous chain |
| 2 | B (B) | Tubulin beta chain | polymer | 445 | 49999.9 | 1 | UniProt (D0VWY9) | Ovis aries (Sheep) | |
| 3 | C (C) | Kinesin-like protein KIF2C,KIF2C FUSED TO A DARPIN,KIF2C FUSED TO A DARPIN | polymer | 573 | 62110.4 | 1 | UniProt (Q99661) Pfam (PF00225) | Homo sapiens (Human) | Kinesin-like protein 6,Mitotic centromere-associated kinesin,MCAK |
| 4 | D (A) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466) | |||
| 5 | E, H (A, C) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) PubChem (888) | |||
| 6 | F (B) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 1 | Chemie (GDP) PubChem (136073927) PubChem (135398619) PubChem (135402030) PubChem (135431654) PubChem (135566337) PubChem (135760556) PubChem (136026707) PubChem (136053418) PubChem (136663964) PubChem (136664748) PubChem (136759848) PubChem (136759849) PubChem (136759850) PubChem (136836636) PubChem (136847074) PubChem (172898537) | |||
| 7 | G (B) | N-[(7S)-1,2,3,10-tetramethoxy-9-oxo-6,7-dihydro-5H-benzo[d]heptalen-7-yl]ethanamide | non-polymer | 399.4 | 1 | Chemie (LOC) PubChem (6167) PubChem (53278) PubChem (2833) | |||
| 8 | I (C) | PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER | non-polymer | 506.2 | 1 | Chemie (ANP) |
Sequence modifications
B: 1 - 455 (UniProt: D0VWY9)
C: 216 - 598 (UniProt: Q99661)
C: 622 - 640 (PDB: 5MIO)
C: 641 - 786 (PDB: 5MIO)
| PDB | External Database | Details |
|---|---|---|
| Cys 203 | Ser 201 | conflict |
| Ile 318 | Val 316 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Met 214 | - | initiating methionine |
| Gly 215 | - | expression tag |
| Ala 330 | Arg 330 | engineered mutation |
| Ala 379 | Arg 379 | engineered mutation |
| Gly 599 | - | linker |
| Gly 600 | - | linker |
| Gly 601 | - | linker |
| Gly 602 | - | linker |
| Ser 603 | - | linker |
| Gly 604 | - | linker |
| Gly 605 | - | linker |
| Gly 606 | - | linker |
| Gly 607 | - | linker |
| Ser 608 | - | linker |
| Gly 609 | - | linker |
| Gly 610 | - | linker |
| Gly 611 | - | linker |
| Gly 612 | - | linker |
| Ser 613 | - | linker |
| Gly 614 | - | linker |
| Gly 615 | - | linker |
| Gly 616 | - | linker |
| Gly 617 | - | linker |
| Ser 618 | - | linker |
| Gly 619 | - | linker |
| Gly 620 | - | linker |
| Ser 621 | - | linker |
C: 641 - 786 (PDB: 5MIO)
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 162314.8 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 1920.6 | |
| All* | Total formula weight | 164235.4 |
