5MI9
Structure of the phosphomimetic mutant of the elongation factor EF-Tu T62E
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Elongation factor Tu 1 | polymer | 402 | 44357.5 | 2 | UniProt (A8A5E6) Pfam (PF00009) Pfam (PF03144) Pfam (PF03143) UniProt (by SIFTS) (P0CE47) | Escherichia coli O9:H4 (strain HS) | EF-Tu 1 |
| 2 | C, E (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 3 | D, F (A, B) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 2 | Chemie (GDP) | |||
| 4 | G, H (A, B) | water | water | 18.0 | 11 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 394 (UniProt: A8A5E6)
| PDB | External Database | Details |
|---|---|---|
| Met -7 | - | initiating methionine |
| Gly -6 | - | expression tag |
| Ser -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| His -1 | - | expression tag |
| His 0 | - | expression tag |
| His 1 | - | expression tag |
| Glu 62 | Thr 62 | engineered mutation |
| Cys 222 | Ser 222 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 88715.1 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 935.0 | |
| All* | Total formula weight | 89650.1 |
