5MI9
Structure of the phosphomimetic mutant of the elongation factor EF-Tu T62E
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Elongation factor Tu 1 | polymer | 402 | 44357.5 | 2 | UniProt (A8A5E6) Pfam (PF00009) Pfam (PF03144) Pfam (PF03143) UniProt (by SIFTS) (P0CE47) | Escherichia coli O9:H4 (strain HS) | EF-Tu 1 |
| 2 | C, E (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) PubChem (888) | |||
| 3 | D, F (A, B) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 2 | Chemie (GDP) PubChem (136073927) PubChem (135398619) PubChem (135402030) PubChem (135431654) PubChem (135566337) PubChem (135760556) PubChem (136026707) PubChem (136053418) PubChem (136663964) PubChem (136664748) PubChem (136759848) PubChem (136759849) PubChem (136759850) PubChem (136836636) PubChem (136847074) PubChem (172898537) | |||
| 4 | G, H (A, B) | water | water | 18.0 | 11 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 394 (UniProt: A8A5E6)
| PDB | External Database | Details |
|---|---|---|
| Met -7 | - | initiating methionine |
| Gly -6 | - | expression tag |
| Ser -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| His -1 | - | expression tag |
| His 0 | - | expression tag |
| His 1 | - | expression tag |
| Glu 62 | Thr 62 | engineered mutation |
| Cys 222 | Ser 222 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 88715.1 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 935.0 | |
| All* | Total formula weight | 89650.1 |
