5MI9
Structure of the phosphomimetic mutant of the elongation factor EF-Tu T62E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-21 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.120, 62.100, 65.470 |
| Unit cell angles | 109.25, 106.00, 88.15 |
Refinement procedure
| Resolution | 51.040 - 3.300 |
| R-factor | 0.2271 |
| Rwork | 0.226 |
| R-free | 0.25290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.280 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 51.040 |
| High resolution limit [Å] | 3.290 |
| Rmeas | 0.147 |
| Number of reflections | 12489 |
| <I/σ(I)> | 6.2 |
| Completeness [%] | 98.0 |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M MgCl2, 0.1 M HEPES and 10 % PEG4000 |
