5MI3

Structure of phosphorylated translation elongation factor EF-Tu from E. coli

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Elongation factor Tu 1	polymer	402	44393.4	2	UniProt (P0CE47) Pfam (PF00009) Pfam (PF03144) Pfam (PF03143)	Escherichia coli (strain K12)	EF-Tu 1,Bacteriophage Q beta RNA-directed RNA polymerase subunit III,P-43
2	C, E (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
3	D, F (A, B)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP)
4	G, H (A, B)	water	water		18.0	244	Chemie (HOH)

Sequence modifications

A, B: 2 - 394 (UniProt: P0CE47)

PDB	External Database	Details
Met -7	-	initiating methionine
Gly -6	-	expression tag
Ser -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	88786.9
Non-Polymers*	Number of molecules	4
	Total formula weight	935.0
All*	Total formula weight	89721.9

*Water molecules are not included.