5MI3

Structure of phosphorylated translation elongation factor EF-Tu from E. coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-02
Detector	DECTRIS PILATUS3 R CdTe 300K
Wavelength(s)	1.000
Spacegroup name	P 2 21 21
Unit cell lengths	68.200, 244.400, 61.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.771 - 2.800
R-factor	0.1556
Rwork	0.153
R-free	0.19840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1efc
RMSD bond length	0.008
RMSD bond angle	1.027
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2152: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	45.800
High resolution limit [Å]	2.800
Rmerge	0.134
Number of reflections	25588
<I/σ(I)>	19.4
Completeness [%]	97.1
Redundancy	7.2
CC(1/2)	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M NaCl, 0.1 M MES and 10 % PEG4000.