5MFR

The crystal structure of E. coli Aminopeptidase N in complex with 7-amino-5,7,8,9-tetrahydrobenzocyclohepten-6-one

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Aminopeptidase N	polymer	891	101441.2	1	UniProt (P04825) Pfam (PF17900) Pfam (PF01433) Pfam (PF11940) Pfam (PF17432)	Escherichia coli (strain K12)	Alpha-aminoacylpeptide hydrolase
2	B (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
3	C (A)	[(7~{S})-6,6-bis(oxidanyl)-5,7,8,9-tetrahydrobenzo[7]annulen-7-yl]azanium	non-polymer		194.3	1	Chemie (7MK)
4	D, E, F, G, H... (A)	GLYCEROL	non-polymer		92.1	10	Chemie (GOL)
5	N, O, P, Q, R... (A)	SODIUM ION	non-polymer		23.0	8	Chemie (NA)
6	V, W, X, Y (A)	MALONATE ION	non-polymer		102.0	4	Chemie (MLI)
7	AA, Z (A)	DIMETHYL SULFOXIDE	non-polymer		78.1	2	Chemie (DMS)
8	BA, CA, DA (A)	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
9	EA (A)	water	water		18.0	1798	Chemie (HOH)

Sequence modifications

A: 1 - 870 (UniProt: P04825)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	101441.2
Non-Polymers*	Number of molecules	29
	Total formula weight	2035.3
All*	Total formula weight	103476.5

*Water molecules are not included.