5MFR
The crystal structure of E. coli Aminopeptidase N in complex with 7-amino-5,7,8,9-tetrahydrobenzocyclohepten-6-one
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004177 | molecular_function | aminopeptidase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0006508 | biological_process | proteolysis |
| A | 0008233 | molecular_function | peptidase activity |
| A | 0008237 | molecular_function | metallopeptidase activity |
| A | 0008270 | molecular_function | zinc ion binding |
| A | 0016285 | molecular_function | alanyl aminopeptidase activity |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0046872 | molecular_function | metal ion binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 5 |
| Details | binding site for residue ZN A 1001 |
| Chain | Residue |
| A | HIS297 |
| A | HIS301 |
| A | GLU320 |
| A | 7MK1002 |
| A | GOL1011 |
| site_id | AC2 |
| Number of Residues | 18 |
| Details | binding site for residue 7MK A 1002 |
| Chain | Residue |
| A | HIS297 |
| A | GLU298 |
| A | HIS301 |
| A | LYS319 |
| A | GLU320 |
| A | TYR381 |
| A | ZN1001 |
| A | GOL1011 |
| A | MLI1021 |
| A | MLI1022 |
| A | HOH1358 |
| A | HOH1672 |
| A | HOH2243 |
| A | HOH2536 |
| A | GLU121 |
| A | MET260 |
| A | ALA262 |
| A | GLU264 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | binding site for residue GOL A 1003 |
| Chain | Residue |
| A | ASN623 |
| A | GLY791 |
| A | ARG832 |
| A | HOH1119 |
| A | HOH1229 |
| A | HOH1232 |
| site_id | AC4 |
| Number of Residues | 8 |
| Details | binding site for residue GOL A 1004 |
| Chain | Residue |
| A | GLU292 |
| A | VAL670 |
| A | HIS672 |
| A | GOL1009 |
| A | HOH1387 |
| A | HOH1869 |
| A | HOH2341 |
| A | HOH2650 |
| site_id | AC5 |
| Number of Residues | 7 |
| Details | binding site for residue GOL A 1005 |
| Chain | Residue |
| A | PHE704 |
| A | ALA707 |
| A | LYS738 |
| A | TRP739 |
| A | HOH1120 |
| A | HOH1267 |
| A | HOH1780 |
| site_id | AC6 |
| Number of Residues | 10 |
| Details | binding site for residue GOL A 1006 |
| Chain | Residue |
| A | LEU61 |
| A | VAL62 |
| A | ARG669 |
| A | GLU671 |
| A | HOH1239 |
| A | HOH1310 |
| A | HOH2074 |
| A | HOH2108 |
| A | HOH2130 |
| A | HOH2789 |
| site_id | AC7 |
| Number of Residues | 10 |
| Details | binding site for residue GOL A 1007 |
| Chain | Residue |
| A | ASP58 |
| A | LEU59 |
| A | GLU97 |
| A | ILE98 |
| A | SER99 |
| A | HOH1492 |
| A | HOH1734 |
| A | HOH1938 |
| A | HOH2081 |
| A | HOH2216 |
| site_id | AC8 |
| Number of Residues | 13 |
| Details | binding site for residue GOL A 1008 |
| Chain | Residue |
| A | HIS11 |
| A | ASP12 |
| A | ARG14 |
| A | ARG468 |
| A | THR469 |
| A | LYS478 |
| A | GLN479 |
| A | HOH1138 |
| A | HOH1401 |
| A | HOH1825 |
| A | HOH1845 |
| A | HOH2289 |
| A | HOH2761 |
| site_id | AC9 |
| Number of Residues | 6 |
| Details | binding site for residue GOL A 1009 |
| Chain | Residue |
| A | TRP74 |
| A | GLU671 |
| A | GOL1004 |
| A | HOH1187 |
| A | HOH1902 |
| A | HOH2789 |
| site_id | AD1 |
| Number of Residues | 10 |
| Details | binding site for residue GOL A 1010 |
| Chain | Residue |
| A | MET368 |
| A | PRO473 |
| A | ASP474 |
| A | ALA476 |
| A | HOH1126 |
| A | HOH1279 |
| A | HOH1395 |
| A | HOH1534 |
| A | HOH1587 |
| A | HOH1913 |
| site_id | AD2 |
| Number of Residues | 15 |
| Details | binding site for residue GOL A 1011 |
| Chain | Residue |
| A | LYS319 |
| A | GLU320 |
| A | TYR381 |
| A | ZN1001 |
| A | 7MK1002 |
| A | MLI1021 |
| A | HOH1358 |
| A | HOH1672 |
| A | HOH2536 |
| A | GLU121 |
| A | ALA262 |
| A | GLU264 |
| A | HIS297 |
| A | GLU298 |
| A | HIS301 |
| site_id | AD3 |
| Number of Residues | 7 |
| Details | binding site for residue GOL A 1012 |
| Chain | Residue |
| A | LYS541 |
| A | GLU627 |
| A | PHE629 |
| A | ASP630 |
| A | ARG835 |
| A | HOH1673 |
| A | HOH2324 |
| site_id | AD4 |
| Number of Residues | 6 |
| Details | binding site for residue NA A 1013 |
| Chain | Residue |
| A | SER332 |
| A | ASP333 |
| A | GLY335 |
| A | HOH1970 |
| A | HOH2209 |
| A | HOH2332 |
| site_id | AD5 |
| Number of Residues | 6 |
| Details | binding site for residue NA A 1014 |
| Chain | Residue |
| A | HIS771 |
| A | PHE774 |
| A | HOH1400 |
| A | HOH1633 |
| A | HOH2196 |
| A | HOH2222 |
| site_id | AD6 |
| Number of Residues | 6 |
| Details | binding site for residue NA A 1015 |
| Chain | Residue |
| A | SER508 |
| A | HOH1924 |
| A | HOH1999 |
| A | HOH2163 |
| A | HOH2359 |
| A | HOH2835 |
| site_id | AD7 |
| Number of Residues | 6 |
| Details | binding site for residue NA A 1016 |
| Chain | Residue |
| A | ASP452 |
| A | HOH1149 |
| A | HOH1263 |
| A | HOH2265 |
| A | HOH2283 |
| A | HOH2692 |
| site_id | AD8 |
| Number of Residues | 7 |
| Details | binding site for residue NA A 1017 |
| Chain | Residue |
| A | GLU123 |
| A | GLU371 |
| A | HOH1159 |
| A | HOH1273 |
| A | HOH1418 |
| A | HOH1597 |
| A | HOH2317 |
| site_id | AD9 |
| Number of Residues | 5 |
| Details | binding site for residue NA A 1018 |
| Chain | Residue |
| A | ASN67 |
| A | THR91 |
| A | HOH2246 |
| A | HOH2394 |
| A | HOH2409 |
| site_id | AE1 |
| Number of Residues | 5 |
| Details | binding site for residue NA A 1019 |
| Chain | Residue |
| A | ASP452 |
| A | TYR544 |
| A | HOH1535 |
| A | HOH2340 |
| A | HOH2504 |
| site_id | AE2 |
| Number of Residues | 6 |
| Details | binding site for residue NA A 1020 |
| Chain | Residue |
| A | MET260 |
| A | ASN373 |
| A | GLN821 |
| A | HOH1591 |
| A | HOH1701 |
| A | HOH2146 |
| site_id | AE3 |
| Number of Residues | 10 |
| Details | binding site for residue MLI A 1021 |
| Chain | Residue |
| A | TYR275 |
| A | ARG293 |
| A | HIS297 |
| A | GLU298 |
| A | 7MK1002 |
| A | GOL1011 |
| A | MLI1022 |
| A | HOH1165 |
| A | HOH1669 |
| A | HOH2536 |
| site_id | AE4 |
| Number of Residues | 9 |
| Details | binding site for residue MLI A 1022 |
| Chain | Residue |
| A | TYR376 |
| A | THR377 |
| A | LEU378 |
| A | TYR381 |
| A | GLU382 |
| A | ARG825 |
| A | 7MK1002 |
| A | MLI1021 |
| A | HOH1165 |
| site_id | AE5 |
| Number of Residues | 10 |
| Details | binding site for residue MLI A 1023 |
| Chain | Residue |
| A | LYS8 |
| A | GLU123 |
| A | TRP313 |
| A | PHE314 |
| A | VAL369 |
| A | MET372 |
| A | HOH1136 |
| A | HOH1159 |
| A | HOH1193 |
| A | HOH1929 |
| site_id | AE6 |
| Number of Residues | 9 |
| Details | binding site for residue MLI A 1024 |
| Chain | Residue |
| A | ASP28 |
| A | LEU29 |
| A | ASP30 |
| A | LYS33 |
| A | VAL35 |
| A | ARG171 |
| A | HOH1116 |
| A | HOH1178 |
| A | HOH2039 |
| site_id | AE7 |
| Number of Residues | 6 |
| Details | binding site for residue DMS A 1025 |
| Chain | Residue |
| A | THR393 |
| A | LEU394 |
| A | LEU395 |
| A | GLY396 |
| A | ASN507 |
| A | HOH1859 |
| site_id | AE8 |
| Number of Residues | 4 |
| Details | binding site for residue DMS A 1026 |
| Chain | Residue |
| A | THR393 |
| A | GLU535 |
| A | HOH1166 |
| A | HOH2131 |
| site_id | AE9 |
| Number of Residues | 4 |
| Details | binding site for residue CL A 1027 |
| Chain | Residue |
| A | ASP216 |
| A | ALA254 |
| A | SER273 |
| A | HOH1661 |
| site_id | AF1 |
| Number of Residues | 3 |
| Details | binding site for residue CL A 1028 |
| Chain | Residue |
| A | ASN560 |
| A | ASP561 |
| A | HOH2466 |
| site_id | AF2 |
| Number of Residues | 3 |
| Details | binding site for residue CL A 1029 |
| Chain | Residue |
| A | ALA31 |
| A | GLN32 |
| A | HOH2039 |
Functional Information from PROSITE/UniProt
| site_id | PS00142 |
| Number of Residues | 10 |
| Details | ZINC_PROTEASE Neutral zinc metallopeptidases, zinc-binding region signature. VIGHEYFHNW |
| Chain | Residue | Details |
| A | VAL294-TRP303 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | Active site: {"description":"Proton acceptor","evidences":[{"source":"PubMed","id":"16885166","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"18416562","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"19622865","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 8 |
| Details | Binding site: {} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | Site: {"description":"Transition state stabilizer","evidences":[{"evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
