5MF1

Crystal structure of a C-terminally truncated trimeric ectodomain of the Chlamydomonas reinhardtii gamete fusion protein HAP2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Fusion protein HAP2/GCS1	polymer	611	64419.8	3	UniProt (A4GRC6) Pfam (PF10699)	Chlamydomonas reinhardtii
2	D, F, H (A, B, C)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)
3	E, G, I (A, B, C)	2-acetamido-2-deoxy-beta-D-galactopyranose	non-polymer		221.2	3	Chemie (NGA)

Sequence modifications

A, B, C: 23 - 592 (UniProt: A4GRC6)

PDB	External Database	Details
Arg 21	-	cloning artifact
Ser 22	-	cloning artifact
Gly 593	-	expression tag
Pro 594	-	expression tag
Phe 595	-	expression tag
Glu 596	-	expression tag
Asp 597	-	expression tag
Asp 598	-	expression tag
Asp 599	-	expression tag
Asp 600	-	expression tag
Lys 601	-	expression tag
Ala 602	-	expression tag
Gly 603	-	expression tag
Trp 604	-	expression tag
Ser 605	-	expression tag
His 606	-	expression tag
Pro 607	-	expression tag
Gln 608	-	expression tag
Phe 609	-	expression tag
Glu 610	-	expression tag
Lys 611	-	expression tag
Gly 612	-	expression tag
Gly 613	-	expression tag
Gly 614	-	expression tag
Ser 615	-	expression tag
Gly 616	-	expression tag
Gly 617	-	expression tag
Gly 618	-	expression tag
Ser 619	-	expression tag
Gly 620	-	expression tag
Gly 621	-	expression tag
Gly 622	-	expression tag
Ser 623	-	expression tag
Trp 624	-	expression tag
Ser 625	-	expression tag
His 626	-	expression tag
Pro 627	-	expression tag
Gln 628	-	expression tag
Phe 629	-	expression tag
Glu 630	-	expression tag
Lys 631	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	193259.4
Non-Polymers*	Number of molecules	6
	Total formula weight	1327.2
All*	Total formula weight	194586.7

*Water molecules are not included.