5MF1
Crystal structure of a C-terminally truncated trimeric ectodomain of the Chlamydomonas reinhardtii gamete fusion protein HAP2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2015-02-05 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.965 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 103.970, 114.060, 137.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.620 - 3.300 |
| R-factor | 0.2213 |
| Rwork | 0.219 |
| R-free | 0.26620 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.120 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | autoSHARP |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.620 | 3.418 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rpim | 0.105 | 0.643 |
| Number of reflections | 25181 | |
| <I/σ(I)> | 7.49 | |
| Completeness [%] | 99.6 | 99.07 |
| Redundancy | 4.86 | 4.9 |
| CC(1/2) | 0.989 | 0.421 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100 mM HEPES pH7.5 2% 2-Propanol 100 mM sodium acetate 12-14%w/v PEG 8000 |
