5M3L
Single-particle cryo-EM using alignment by classification (ABC): the structure of Lumbricus terrestris hemoglobin
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, E, I (A, E, I) | Extracellular globin-4 | polymer | 151 | 17567.0 | 3 | UniProt (P13579) Pfam (PF00042) | Lumbricus terrestris (Common earthworm) | Erythrocruorin,Globin A,Globin IV |
| 2 | B, F, J (B, F, J) | Extracellular globin-2 | polymer | 145 | 16268.2 | 3 | UniProt (P02218) Pfam (PF00042) | Lumbricus terrestris (Common earthworm) | Erythrocruorin,Globin AIII,Globin B,Globin II |
| 3 | C, G, K (C, G, K) | Extracellular globin-3 | polymer | 153 | 17331.8 | 3 | UniProt (P11069) Pfam (PF00042) | Lumbricus terrestris (Common earthworm) | Erythrocruorin,Extracellular globin III,Globin C |
| 4 | D, H, L (D, H, L) | Hemoglobin chain d1 | polymer | 140 | 15988.3 | 3 | UniProt (O61233) Pfam (PF00042) | Lumbricus terrestris (Common earthworm) | |
| 5 | M (M) | Hemoglobin linker chain L1 | polymer | 217 | 24936.7 | 1 | UniProt (Q9GV76) Pfam (PF00057) Pfam (PF16915) | Lumbricus terrestris (Common earthworm) | |
| 6 | N (N) | Extracellular hemoglobin linker L2 subunit | polymer | 220 | 25085.0 | 1 | UniProt (Q2I743) Pfam (PF00057) Pfam (PF16915) | Lumbricus terrestris (Common earthworm) | |
| 7 | O (O) | Extracellular hemoglobin linker L3 subunit | polymer | 215 | 24486.0 | 1 | UniProt (Q2I742) Pfam (PF16915) Pfam (PF00057) | Lumbricus terrestris (Common earthworm) | |
| 8 | AA, P, Q, R, S... (L, A, B, C, D...) | PROTOPORPHYRIN IX CONTAINING FE | non-polymer | 616.5 | 12 | Chemie (HEM) |
Sequence modifications
A, E, I: 1 - 151 (UniProt: P13579)
B, F, J: 1 - 145 (UniProt: P02218)
C, G, K: 1 - 153 (UniProt: P11069)
N: 10 - 229 (UniProt: Q2I743)
O: 8 - 222 (UniProt: Q2I742)
| PDB | External Database | Details |
|---|---|---|
| Lys 78 | Asp 78 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Asp 66 | Glu 66 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Glu 49 | Asp 66 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Glu 55 | Thr 92 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ile 17 | Leu 35 | conflict |
| Cys 113 | Val 131 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 15 |
| Total formula weight | 275973.6 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 7397.8 | |
| All* | Total formula weight | 283371.5 |
