5M3B

Structure of cobinamide-bound BtuF mutant W66L, the periplasmic vitamin B12 binding protein in E.coli

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Vitamin B12-binding protein	polymer	289	31438.9	2	UniProt (P37028) Pfam (PF01497)	Escherichia coli
2	C, E, H (A, B)	CYANIDE ION	non-polymer		26.0	3	Chemie (CYN)
3	D, G (A, B)	COB(II)INAMIDE	non-polymer		990.1	2	Chemie (CBY)
4	F, I, J (A, B)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
5	K, L (A, B)	water	water		18.0	555	Chemie (HOH)

Sequence modifications

A, B: 22 - 266 (UniProt: P37028)

PDB	External Database	Details
Met -2	-	initiating methionine
Lys -1	-	expression tag
Lys 0	-	expression tag
Thr 1	-	expression tag
Ala 2	-	expression tag
Ile 3	-	expression tag
Ala 4	-	expression tag
Ile 5	-	expression tag
Ala 6	-	expression tag
Val 7	-	expression tag
Ala 8	-	expression tag
Leu 9	-	expression tag
Ala 10	-	expression tag
Gly 11	-	expression tag
Phe 12	-	expression tag
Ala 13	-	expression tag
Thr 14	-	expression tag
Val 15	-	expression tag
Ala 16	-	expression tag
Gln 17	-	expression tag
Ala 18	-	expression tag
Ala 19	-	expression tag
Ser 20	-	expression tag
Met 21	-	expression tag
Leu 66	Trp 66	engineered mutation
Ser 267	-	expression tag
Gly 268	-	expression tag
Ser 269	-	expression tag
Leu 270	-	expression tag
Glu 271	-	expression tag
Val 272	-	expression tag
Leu 273	-	expression tag
Phe 274	-	expression tag
Gln 275	-	expression tag
Gly 276	-	expression tag
Pro 277	-	expression tag
Gly 278	-	expression tag
Gly 279	-	expression tag
Ser 280	-	expression tag
His 281	-	expression tag
His 282	-	expression tag
His 283	-	expression tag
His 284	-	expression tag
His 285	-	expression tag
His 286	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	62877.7
Non-Polymers*	Number of molecules	8
	Total formula weight	2334.5
All*	Total formula weight	65212.3

*Water molecules are not included.