5M0J

Crystal structure of the cytoplasmic complex with She2p, She3p, and the ASH1 mRNA E3-localization element

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	D, C, B, A, J...	SWI5-dependent HO expression protein 2,SWI5-dependent HO expression protein 3	polymer	328	36130.5	8	UniProt (B3LQW9) UniProt (B3LN26) Pfam (PF11435) UniProt (by SIFTS) (P36068) UniProt (by SIFTS) (P38272) In PDB	Saccharomyces cerevisiae (strain RM11-1a) (Baker's yeast)
2	E, F	ASH1 E3 (28 nt-loop)	polymer	28	8994.4	2		Saccharomyces cerevisiae
3	D, C, B, A	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
4		water	water		18.0	207	Chemie (HOH)

Sequence modifications

D, C, B, A, J, G, H, I: 6 - 246 (UniProt: B3LQW9)

PDB	External Database	Details
Gly 4	-	expression tag
Pro 5	-	expression tag
Ser 14	Cys 14	engineered mutation
Ser 68	Cys 68	engineered mutation
Ser 106	Cys 106	engineered mutation
Ser 180	Cys 180	engineered mutation
Gly 247	-	linker
Gly 248	-	linker
Ser 249	-	linker
Gly 250	-	linker
Gly 251	-	linker
Gly 252	-	linker
Gly 253	-	linker
Ser 254	-	linker
Gly 255	-	linker
Gly 256	-	linker

D, C, B, A, J, G, H, I: 257 - 331 (UniProt: B3LN26)

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	307032.8
Non-Polymers*	Number of molecules	4
	Total formula weight	97.2
All*	Total formula weight	307130.0

*Water molecules are not included.