5M0J
Crystal structure of the cytoplasmic complex with She2p, She3p, and the ASH1 mRNA E3-localization element
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-01 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.9175 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 219.030, 58.980, 144.680 |
| Unit cell angles | 90.00, 126.91, 90.00 |
Refinement procedure
| Resolution | 50.010 - 2.800 |
| R-factor | 0.19701 |
| Rwork | 0.194 |
| R-free | 0.24614 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xly |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.451 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.189 | 0.800 |
| Number of reflections | 35077 | |
| <I/σ(I)> | 10.34 | 2.02 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 6.8 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 20 % (w/v) PEG3350, 150 mM NaNO3 |
