5M0I
Crystal structure of the nuclear complex with She2p and the ASH1 mRNA E3-localization element
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (D, C, B, A) | SWI5-dependent HO expression protein 2 | polymer | 246 | 28037.7 | 4 | UniProt (B3LQW9) Pfam (PF11435) UniProt (by SIFTS) (P36068) | Saccharomyces cerevisiae | |
| 2 | E, F (E, F) | ASH1-E3 element, RNA (28-MER) | polymer | 28 | 8994.4 | 2 | Saccharomyces cerevisiae | ||
| 3 | G, H, I (H, J, I) | SWI5-dependent HO expression protein 3 | polymer | 24 | 2522.7 | 3 | UniProt (P38272) | Saccharomyces cerevisiae S288c | |
| 4 | J, K, N, O, P... (D, C, B, A, E) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 11 | Chemie (EDO) | |||
| 5 | DA, EA, L, T, V... (F, D, C, B, A) | MAGNESIUM ION | non-polymer | 24.3 | 6 | Chemie (MG) | |||
| 6 | AA, BA, CA, FA, M... (E, F, D, B) | ACETATE ION | non-polymer | 59.0 | 6 | Chemie (ACT) | |||
| 7 | GA, HA, IA, JA, KA... (D, C, B, A, E...) | water | water | 18.0 | 333 | Chemie (HOH) |
Sequence modifications
D, C, B, A: 6 - 246 (UniProt: B3LQW9)
| PDB | External Database | Details |
|---|---|---|
| Gly 1 | - | expression tag |
| Pro 2 | - | expression tag |
| Leu 3 | - | expression tag |
| Gly 4 | - | expression tag |
| Ser 5 | - | expression tag |
| Ser 14 | Cys 14 | engineered mutation |
| Ser 68 | Cys 68 | engineered mutation |
| Ser 106 | Cys 106 | engineered mutation |
| Ser 180 | Cys 180 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 137708.1 | |
| Non-Polymers* | Number of molecules | 23 |
| Total formula weight | 1182.8 | |
| All* | Total formula weight | 138890.9 |
