5LTA
Crystal structure of the Prp43-ADP-BeF3-U7-RNA complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43 | polymer | 714 | 81641.3 | 1 | UniProt (G0RY84) Pfam (PF00270) Pfam (PF00271) Pfam (PF04408) Pfam (PF21010) Pfam (PF07717) | Chaetomium thermophilum var. thermophilum DSM 1495 | |
| 2 | B (E) | RNA (5'-R(P*UP*UP*UP*UP*UP*UP*UP*U)-3') | polymer | 16 | 4853.7 | 1 | synthetic construct | ||
| 3 | C (A) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 1 | Chemie (ADP) | |||
| 4 | D (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 5 | E (A) | BERYLLIUM TRIFLUORIDE ION | non-polymer | 66.0 | 1 | Chemie (BEF) | |||
| 6 | F (A) | CACODYLATE ION | non-polymer | 137.0 | 1 | Chemie (CAC) | |||
| 7 | G, H (A, E) | water | water | 18.0 | 52 | Chemie (HOH) |
Sequence modifications
A: 61 - 764 (UniProt: G0RY84)
| PDB | External Database | Details |
|---|---|---|
| Met 59 | - | initiating methionine |
| Ala 60 | - | expression tag |
| Trp 765 | - | expression tag |
| Ser 766 | - | expression tag |
| His 767 | - | expression tag |
| Pro 768 | - | expression tag |
| Gln 769 | - | expression tag |
| Phe 770 | - | expression tag |
| Glu 771 | - | expression tag |
| Lys 772 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 86495.0 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 654.5 | |
| All* | Total formula weight | 87149.5 |
