5LTA

Crystal structure of the Prp43-ADP-BeF3-U7-RNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-19
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.82656
Spacegroup name	P 61 2 2
Unit cell lengths	106.392, 106.392, 356.698
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.558 - 2.621
R-factor	0.1989
Rwork	0.197
R-free	0.22950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5d0u
RMSD bond length	0.003
RMSD bond angle	0.799
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.560	2.700
High resolution limit [Å]	2.620	2.620
Rmerge	0.059	0.900
Number of reflections	36887
<I/σ(I)>	22.16	1.85
Completeness [%]	98.5	86.8
Redundancy	5.14	5.34
CC(1/2)	0.999	0.652

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	2.5 % (w/v) PGA-LM, 13 % (w/v) PEG 8000 and 100 mM Na Cacodylate pH 6.5