5LMM
Structure of E coli Hydrogenase Hyd-1 mutant E28Q
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (S, T) | Hydrogenase-1 small chain | polymer | 335 | 36814.7 | 2 | UniProt (P69740) Pfam (PF01058) Pfam (PF14720) UniProt (by SIFTS) (P69739) | Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) | HYD1,Membrane-bound hydrogenase 1 small subunit,NiFe hydrogenase |
| 2 | B, D (L, M) | Hydrogenase-1 large chain | polymer | 582 | 64766.4 | 2 | UniProt (P0ACD8) Pfam (PF00374) | Escherichia coli (strain K12) | HYD1,Membrane-bound hydrogenase 1 large subunit,NiFe hydrogenase |
| 3 | E, P (S, T) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 2 | Chemie (SF4) | |||
| 4 | F, Q (S, T) | FE3-S4 CLUSTER | non-polymer | 295.8 | 2 | Chemie (F3S) | |||
| 5 | G, R (S, T) | FE4-S3 CLUSTER | non-polymer | 319.6 | 2 | Chemie (SF3) | |||
| 6 | H, S (S, T) | DODECYL-BETA-D-MALTOSIDE | non-polymer | 510.6 | 2 | Chemie (LMT) | |||
| 7 | I, T, U (S, T) | CHLORIDE ION | non-polymer | 35.5 | 3 | Chemie (CL) | |||
| 8 | J, K, V, Z (S, L, T, M) | SULFATE ION | non-polymer | 96.1 | 4 | Chemie (SO4) | |||
| 9 | L, W (L, M) | CARBONMONOXIDE-(DICYANO) IRON | non-polymer | 135.9 | 2 | Chemie (FCO) | |||
| 10 | M, X (L, M) | NICKEL (II) ION | non-polymer | 58.7 | 2 | Chemie (NI) | |||
| 11 | N, Y (L, M) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 12 | AA, O (M, L) | LITHIUM ION | non-polymer | 6.9 | 2 | Chemie (LI) | |||
| 13 | BA (M) | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 1 | Chemie (TRS) | |||
| 14 | CA, DA, EA, FA (S, L, T, M) | water | water | 18.0 | 1426 | Chemie (HOH) |
Sequence modifications
S, T: 1 - 327 (UniProt: P69740)
L, M: 1 - 582 (UniProt: P0ACD8)
| PDB | External Database | Details |
|---|---|---|
| Arg 328 | - | expression tag |
| Ser 329 | - | expression tag |
| His 330 | - | expression tag |
| His 331 | - | expression tag |
| His 332 | - | expression tag |
| His 333 | - | expression tag |
| His 334 | - | expression tag |
| His 335 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gln 28 | Glu 28 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 203162.2 | |
| Non-Polymers* | Number of molecules | 24 |
| Total formula weight | 4019.7 | |
| All* | Total formula weight | 207181.8 |
