5LMM
Structure of E coli Hydrogenase Hyd-1 mutant E28Q
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| L | 0005515 | molecular_function | protein binding |
| L | 0005886 | cellular_component | plasma membrane |
| L | 0006113 | biological_process | fermentation |
| L | 0008901 | molecular_function | ferredoxin hydrogenase activity |
| L | 0009055 | molecular_function | electron transfer activity |
| L | 0009061 | biological_process | anaerobic respiration |
| L | 0009267 | biological_process | cellular response to starvation |
| L | 0016020 | cellular_component | membrane |
| L | 0016151 | molecular_function | nickel cation binding |
| L | 0016491 | molecular_function | oxidoreductase activity |
| L | 0019645 | biological_process | anaerobic electron transport chain |
| L | 0030288 | cellular_component | outer membrane-bounded periplasmic space |
| L | 0033748 | molecular_function | hydrogenase (acceptor) activity |
| L | 0044569 | cellular_component | [Ni-Fe] hydrogenase complex |
| L | 0046872 | molecular_function | metal ion binding |
| L | 0098567 | cellular_component | periplasmic side of plasma membrane |
| L | 1902421 | biological_process | hydrogen metabolic process |
| M | 0005515 | molecular_function | protein binding |
| M | 0005886 | cellular_component | plasma membrane |
| M | 0006113 | biological_process | fermentation |
| M | 0008901 | molecular_function | ferredoxin hydrogenase activity |
| M | 0009055 | molecular_function | electron transfer activity |
| M | 0009061 | biological_process | anaerobic respiration |
| M | 0009267 | biological_process | cellular response to starvation |
| M | 0016020 | cellular_component | membrane |
| M | 0016151 | molecular_function | nickel cation binding |
| M | 0016491 | molecular_function | oxidoreductase activity |
| M | 0019645 | biological_process | anaerobic electron transport chain |
| M | 0030288 | cellular_component | outer membrane-bounded periplasmic space |
| M | 0033748 | molecular_function | hydrogenase (acceptor) activity |
| M | 0044569 | cellular_component | [Ni-Fe] hydrogenase complex |
| M | 0046872 | molecular_function | metal ion binding |
| M | 0098567 | cellular_component | periplasmic side of plasma membrane |
| M | 1902421 | biological_process | hydrogen metabolic process |
| S | 0005515 | molecular_function | protein binding |
| S | 0005886 | cellular_component | plasma membrane |
| S | 0006113 | biological_process | fermentation |
| S | 0008901 | molecular_function | ferredoxin hydrogenase activity |
| S | 0009055 | molecular_function | electron transfer activity |
| S | 0009061 | biological_process | anaerobic respiration |
| S | 0009267 | biological_process | cellular response to starvation |
| S | 0009375 | cellular_component | ferredoxin hydrogenase complex |
| S | 0016020 | cellular_component | membrane |
| S | 0016491 | molecular_function | oxidoreductase activity |
| S | 0019645 | biological_process | anaerobic electron transport chain |
| S | 0033748 | molecular_function | hydrogenase (acceptor) activity |
| S | 0044569 | cellular_component | [Ni-Fe] hydrogenase complex |
| S | 0046872 | molecular_function | metal ion binding |
| S | 0051536 | molecular_function | iron-sulfur cluster binding |
| S | 0051538 | molecular_function | 3 iron, 4 sulfur cluster binding |
| S | 0051539 | molecular_function | 4 iron, 4 sulfur cluster binding |
| S | 0098567 | cellular_component | periplasmic side of plasma membrane |
| S | 1902421 | biological_process | hydrogen metabolic process |
| T | 0005515 | molecular_function | protein binding |
| T | 0005886 | cellular_component | plasma membrane |
| T | 0006113 | biological_process | fermentation |
| T | 0008901 | molecular_function | ferredoxin hydrogenase activity |
| T | 0009055 | molecular_function | electron transfer activity |
| T | 0009061 | biological_process | anaerobic respiration |
| T | 0009267 | biological_process | cellular response to starvation |
| T | 0009375 | cellular_component | ferredoxin hydrogenase complex |
| T | 0016020 | cellular_component | membrane |
| T | 0016491 | molecular_function | oxidoreductase activity |
| T | 0019645 | biological_process | anaerobic electron transport chain |
| T | 0033748 | molecular_function | hydrogenase (acceptor) activity |
| T | 0044569 | cellular_component | [Ni-Fe] hydrogenase complex |
| T | 0046872 | molecular_function | metal ion binding |
| T | 0051536 | molecular_function | iron-sulfur cluster binding |
| T | 0051538 | molecular_function | 3 iron, 4 sulfur cluster binding |
| T | 0051539 | molecular_function | 4 iron, 4 sulfur cluster binding |
| T | 0098567 | cellular_component | periplasmic side of plasma membrane |
| T | 1902421 | biological_process | hydrogen metabolic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 7 |
| Details | binding site for residue SF4 S 401 |
| Chain | Residue |
| S | HIS187 |
| S | CYS190 |
| S | ARG193 |
| S | CYS215 |
| S | LEU216 |
| S | CYS221 |
| S | ILE243 |
| site_id | AC2 |
| Number of Residues | 8 |
| Details | binding site for residue F3S S 402 |
| Chain | Residue |
| S | ASN228 |
| S | CYS230 |
| S | TRP235 |
| S | CYS249 |
| S | LEU250 |
| S | CYS252 |
| L | LYS226 |
| S | THR226 |
| site_id | AC3 |
| Number of Residues | 13 |
| Details | binding site for residue SF3 S 403 |
| Chain | Residue |
| L | HOH726 |
| S | GLU16 |
| S | CYS17 |
| S | THR18 |
| S | CYS19 |
| S | CYS20 |
| S | GLU76 |
| S | THR114 |
| S | CYS115 |
| S | CYS120 |
| S | GLY148 |
| S | CYS149 |
| S | HOH526 |
| site_id | AC4 |
| Number of Residues | 4 |
| Details | binding site for residue LMT S 404 |
| Chain | Residue |
| S | ILE7 |
| S | TRP11 |
| S | MET163 |
| S | HOH678 |
| site_id | AC5 |
| Number of Residues | 4 |
| Details | binding site for residue CL S 405 |
| Chain | Residue |
| S | TRP118 |
| S | CYS120 |
| S | GLY256 |
| S | HOH604 |
| site_id | AC6 |
| Number of Residues | 8 |
| Details | binding site for residue SO4 S 406 |
| Chain | Residue |
| M | ARG355 |
| M | TRP373 |
| M | HOH1094 |
| S | ARG125 |
| S | PRO126 |
| S | HOH510 |
| S | HOH557 |
| S | HOH671 |
| site_id | AC7 |
| Number of Residues | 3 |
| Details | binding site for residue SO4 L 601 |
| Chain | Residue |
| L | LEU482 |
| L | ALA483 |
| S | ARG211 |
| site_id | AC8 |
| Number of Residues | 13 |
| Details | binding site for residue FCO L 602 |
| Chain | Residue |
| L | CSO79 |
| L | VAL82 |
| L | HIS83 |
| L | ALA507 |
| L | PRO508 |
| L | ARG509 |
| L | LEU512 |
| L | VAL530 |
| L | PRO531 |
| L | THR532 |
| L | CYS579 |
| L | NI603 |
| L | HOH1030 |
| site_id | AC9 |
| Number of Residues | 6 |
| Details | binding site for residue NI L 603 |
| Chain | Residue |
| L | CYS76 |
| L | CSO79 |
| L | CYS576 |
| L | CYS579 |
| L | FCO602 |
| L | HOH1030 |
| site_id | AD1 |
| Number of Residues | 6 |
| Details | binding site for residue MG L 604 |
| Chain | Residue |
| L | GLU57 |
| L | CYS528 |
| L | HIS582 |
| L | HOH735 |
| L | HOH748 |
| L | HOH749 |
| site_id | AD2 |
| Number of Residues | 5 |
| Details | binding site for residue LI L 605 |
| Chain | Residue |
| L | ILE97 |
| L | HOH826 |
| L | HOH908 |
| L | HOH986 |
| L | HOH1099 |
| site_id | AD3 |
| Number of Residues | 6 |
| Details | binding site for residue SF4 T 401 |
| Chain | Residue |
| T | HIS187 |
| T | CYS190 |
| T | ARG193 |
| T | CYS215 |
| T | LEU216 |
| T | CYS221 |
| site_id | AD4 |
| Number of Residues | 9 |
| Details | binding site for residue F3S T 402 |
| Chain | Residue |
| M | LYS226 |
| T | THR226 |
| T | ASN228 |
| T | CYS230 |
| T | TRP235 |
| T | PRO242 |
| T | CYS249 |
| T | LEU250 |
| T | CYS252 |
| site_id | AD5 |
| Number of Residues | 13 |
| Details | binding site for residue SF3 T 403 |
| Chain | Residue |
| T | THR114 |
| T | CYS115 |
| T | CYS120 |
| T | GLY148 |
| T | CYS149 |
| T | HOH555 |
| M | HOH717 |
| T | GLU16 |
| T | CYS17 |
| T | THR18 |
| T | CYS19 |
| T | CYS20 |
| T | GLU76 |
| site_id | AD6 |
| Number of Residues | 3 |
| Details | binding site for residue LMT T 404 |
| Chain | Residue |
| T | ILE7 |
| T | TRP11 |
| T | MET163 |
| site_id | AD7 |
| Number of Residues | 5 |
| Details | binding site for residue CL T 405 |
| Chain | Residue |
| T | TRP118 |
| T | CYS120 |
| T | GLY256 |
| T | HOH631 |
| T | HOH682 |
| site_id | AD8 |
| Number of Residues | 5 |
| Details | binding site for residue CL T 406 |
| Chain | Residue |
| T | ILE12 |
| T | HIS13 |
| T | ASP46 |
| T | HOH579 |
| T | HOH655 |
| site_id | AD9 |
| Number of Residues | 2 |
| Details | binding site for residue SO4 T 407 |
| Chain | Residue |
| T | ASP173 |
| T | ARG174 |
| site_id | AE1 |
| Number of Residues | 13 |
| Details | binding site for residue FCO M 601 |
| Chain | Residue |
| M | CSO79 |
| M | VAL82 |
| M | HIS83 |
| M | ALA507 |
| M | PRO508 |
| M | ARG509 |
| M | LEU512 |
| M | VAL530 |
| M | PRO531 |
| M | THR532 |
| M | CYS579 |
| M | NI602 |
| M | HOH994 |
| site_id | AE2 |
| Number of Residues | 6 |
| Details | binding site for residue NI M 602 |
| Chain | Residue |
| M | CYS76 |
| M | CSO79 |
| M | CYS576 |
| M | CYS579 |
| M | FCO601 |
| M | HOH994 |
| site_id | AE3 |
| Number of Residues | 6 |
| Details | binding site for residue MG M 603 |
| Chain | Residue |
| M | GLU57 |
| M | CYS528 |
| M | HIS582 |
| M | HOH726 |
| M | HOH727 |
| M | HOH729 |
| site_id | AE4 |
| Number of Residues | 3 |
| Details | binding site for residue SO4 M 604 |
| Chain | Residue |
| M | ARG394 |
| M | HOH704 |
| S | ARG125 |
| site_id | AE5 |
| Number of Residues | 4 |
| Details | binding site for residue LI M 605 |
| Chain | Residue |
| M | HOH810 |
| M | HOH889 |
| M | HOH932 |
| M | HOH1110 |
| site_id | AE6 |
| Number of Residues | 8 |
| Details | binding site for residue TRS M 606 |
| Chain | Residue |
| M | ASP350 |
| M | ASP359 |
| M | GLN392 |
| M | GLU393 |
| M | ARG394 |
| M | HOH704 |
| M | HOH728 |
| M | HOH867 |
Functional Information from PROSITE/UniProt
| site_id | PS00507 |
| Number of Residues | 26 |
| Details | NI_HGENASE_L_1 Nickel-dependent hydrogenases large subunit signature 1. RGLEiilqgrdprdawafvERiCGVC |
| Chain | Residue | Details |
| L | ARG54-CSO79 |
| site_id | PS00508 |
| Number of Residues | 10 |
| Details | NI_HGENASE_L_2 Nickel-dependent hydrogenases large subunit signature 2. FDPCLACst.H |
| Chain | Residue | Details |
| L | PHE573-HIS582 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 8 |
| Details | BINDING: BINDING => ECO:0000255 |
| Chain | Residue | Details |
| L | CYS76 | |
| L | CSO79 | |
| L | CYS576 | |
| L | CYS579 | |
| M | CYS76 | |
| M | CSO79 | |
| M | CYS576 | |
| M | CYS579 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 84 |
| Details | TRANSMEM: Helical => ECO:0000255 |
| Chain | Residue | Details |
| S | VAL282-ALA302 | |
| T | VAL282-ALA302 | |
| S | THR281-VAL303 | |
| T | THR281-VAL303 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 94 |
| Details | TOPO_DOM: Cytoplasmic => ECO:0000255 |
| Chain | Residue | Details |
| S | VAL303-ALA327 | |
| T | VAL303-ALA327 | |
| S | ASP304-ALA327 | |
| T | ASP304-ALA327 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 22 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:P21853 |
| Chain | Residue | Details |
| S | CYS230 | |
| S | CYS249 | |
| S | CYS252 | |
| T | CYS17 | |
| T | CYS20 | |
| T | CYS115 | |
| T | CYS149 | |
| T | HIS187 | |
| T | CYS190 | |
| T | CYS215 | |
| T | CYS221 | |
| T | CYS230 | |
| T | CYS249 | |
| T | CYS252 | |
| S | CYS17 | |
| S | CYS20 | |
| S | CYS115 | |
| S | CYS149 | |
| S | HIS187 | |
| S | CYS190 | |
| S | CYS215 | |
| S | CYS221 |
