5LM7

Crystal structure of the lambda N-Nus factor complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, F (A, C)	Transcription termination/antitermination protein NusA	polymer	428	47666.6	2	UniProt (P0AFF8) Pfam (PF08529) Pfam (PF00575) Pfam (PF13184) UniProt (by SIFTS) (P0AFF6)	Escherichia coli O157:H7
2	B, G (B, D)	N utilization substance protein B homolog	polymer	141	15838.2	2	UniProt (B7MD74) Pfam (PF01029) UniProt (by SIFTS) (P0A780)	Escherichia coli O45:K1 (strain S88 / ExPEC)	Protein NusB
3	C, H (J, E)	30S ribosomal protein S10	polymer	108	12167.1	2	UniProt (B7MCT6) Pfam (PF00338) UniProt (by SIFTS) (P0A7R5)	Escherichia coli O45:K1 (strain S88 / ExPEC)
4	D, I (N, F)	Antitermination protein N	polymer	89	10279.8	2	UniProt (P03045) Pfam (PF11438)	Enterobacteria phage lambda	Regulatory protein N,PN
5	E, J (R, G)	RNA (29-MER)	polymer	30	9573.7	2		Enterobacteria phage lambda

Sequence modifications

A, C: 1 - 426 (UniProt: P0AFF8)

PDB	External Database	Details
Gly -1	-	expression tag
Ala 0	-	expression tag

B, D: 1 - 139 (UniProt: B7MD74)

PDB	External Database	Details
Gly -1	-	expression tag
Ala 0	-	expression tag

J, E: 1 - 103 (UniProt: B7MCT6)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Leu -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

N, F: 1 - 84 (UniProt: P03045)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Leu -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	191050.8
All*	Total formula weight	191050.8

*Water molecules are not included.