5LM7

Crystal structure of the lambda N-Nus factor complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-29
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.918
Spacegroup name	P 21 21 21
Unit cell lengths	95.776, 101.803, 279.918
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.520 - 3.350
R-factor	0.2986
Rwork	0.297
R-free	0.33540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l2f 2kwp 1u9l 3b3d 1qfq
RMSD bond length	0.004
RMSD bond angle	0.845
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.550
High resolution limit [Å]	3.350	3.350
Number of reflections	40077
<I/σ(I)>	11.56
Completeness [%]	99.3	98.3
Redundancy	7.3	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1M HEPES pH 7.5, 40%(v/v) ethylene glycol, 5% (w/v) PEG 3000