5LKY
X-ray crystal structure of N-acetylneuraminic acid lyase in complex with pyruvate, with the phenylalanine at position 190 replaced with the non-canonical amino acid dihydroxypropylcysteine.
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D | N-acetylneuraminate lyase | polymer | 300 | 34133.5 | 4 | UniProt (Q2G160) Pfam (PF00701) In PDB | Staphylococcus aureus (strain NCTC 8325) | Neu5Ac lyase,N-acetylneuraminate pyruvate-lyase,N-acetylneuraminic acid aldolase,Sialate lyase,Sialic acid aldolase,Sialic acid lyase |
2 | A, B | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 2 | Chemie (PEG) | |||
3 | water | water | 18.0 | 414 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 2 - 293 (UniProt: Q2G160)
PDB | External Database | Details |
---|---|---|
Met -6 | - | initiating methionine |
Glu -5 | - | expression tag |
His -4 | - | expression tag |
His -3 | - | expression tag |
His -2 | - | expression tag |
His -1 | - | expression tag |
His 0 | - | expression tag |
His 1 | - | expression tag |
P9s 190 | Phe 190 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 136534.2 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 212.2 | |
All* | Total formula weight | 136746.4 |