5LKY

X-ray crystal structure of N-acetylneuraminic acid lyase in complex with pyruvate, with the phenylalanine at position 190 replaced with the non-canonical amino acid dihydroxypropylcysteine.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	N-acetylneuraminate lyase	polymer	300	34133.5	4	UniProt (Q2G160) Pfam (PF00701)	Staphylococcus aureus (strain NCTC 8325)	Neu5Ac lyase,N-acetylneuraminate pyruvate-lyase,N-acetylneuraminic acid aldolase,Sialate lyase,Sialic acid aldolase,Sialic acid lyase
2	E, F (A, B)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
3	G, H, I, J (A, B, C, D)	water	water		18.0	414	Chemie (HOH)

Sequence modifications

A, B, C, D: 2 - 293 (UniProt: Q2G160)

PDB	External Database	Details
Met -6	-	initiating methionine
Glu -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
P9s 190	Phe 190	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	136534.2
Non-Polymers*	Number of molecules	2
	Total formula weight	212.2
All*	Total formula weight	136746.4

*Water molecules are not included.