5LJ5
Overall structure of the yeast spliceosome immediately after branching.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (U) | U5 snRNA (small nuclear RNA) | polymer | 179 | 57444.9 | 1 | GenBank (1039023403) | Saccharomyces cerevisiae | |
| 2 | B (E) | Exon 1 (5' exon) of UBC4 pre-mRNA | polymer | 16 | 5170.2 | 1 | Saccharomyces cerevisiae | ||
| 3 | C (I) | Intron of UBC4 pre-mRNA | polymer | 76 | 24109.1 | 1 | GenBank (4718) | Saccharomyces cerevisiae | |
| 4 | D (Z) | U2 snRNA (small nuclear RNA) | polymer | 1175 | 376267.4 | 1 | GenBank (536627) | Saccharomyces cerevisiae | |
| 5 | E (V) | U6 snRNA (small nuclear RNA) | polymer | 112 | 35883.2 | 1 | GenBank (1039022925) | Saccharomyces cerevisiae | |
| 6 | F (A) | Pre-mRNA-splicing factor 8 | polymer | 2413 | 279867.5 | 1 | UniProt (P33334) Pfam (PF08082) Pfam (PF08083) Pfam (PF10598) Pfam (PF10597) Pfam (PF10596) Pfam (PF12134) Pfam (PF08084) | Saccharomyces cerevisiae | |
| 7 | G (B) | Pre-mRNA-splicing helicase BRR2 | polymer | 2163 | 246470.3 | 1 | UniProt (P32639) Pfam (PF00270) Pfam (PF00271) Pfam (PF23445) Pfam (PF02889) Pfam (PF21188) Pfam (PF18149) | Saccharomyces cerevisiae | Protein Snu246 |
| 8 | H (D) | Protein CWC16 | polymer | 278 | 32371.1 | 1 | UniProt (P28320) Pfam (PF04502) | Saccharomyces cerevisiae | |
| 9 | I (F) | Pre-mRNA-splicing factor CWC25 | polymer | 179 | 20412.5 | 1 | UniProt (P53854) Pfam (PF10197) Pfam (PF12542) | Saccharomyces cerevisiae | Complexed with CEF1 protein 25 |
| 10 | J (C) | Pre-mRNA-splicing factor SNU114 | polymer | 1008 | 114174.0 | 1 | UniProt (P36048) Pfam (PF16004) Pfam (PF00009) Pfam (PF03764) Pfam (PF00679) | Saccharomyces cerevisiae | 114 kDa U5 small nuclear ribonucleoprotein component,Growth inhibitory protein 10 |
| 11 | K (G) | Pre-mRNA-splicing factor ISY1 | polymer | 235 | 28073.8 | 1 | UniProt (P21374) Pfam (PF06246) | Saccharomyces cerevisiae | Interactor of SYF1,PRP19-associated complex protein 30 |
| 12 | L (H) | CWC22 | polymer | 591 | 69119.0 | 1 | UniProt (A0A162ERE7) | Saccharomyces cerevisiae | |
| 13 | M (J) | Pre-mRNA-splicing factor PRP46 | polymer | 451 | 50771.3 | 1 | UniProt (Q12417) Pfam (PF00400) | Saccharomyces cerevisiae | Complexed with CEF1 protein 1,PRP nineteen-associated complex protein 50,PRP19-associated complex protein 50,Pre-mRNA-processing protein 46 |
| 14 | N (K) | Pre-mRNA-processing protein 45 | polymer | 379 | 42548.7 | 1 | UniProt (P28004) Pfam (PF02731) | Saccharomyces cerevisiae | |
| 15 | O (L) | Pre-mRNA-splicing factor BUD31 | polymer | 157 | 18484.5 | 1 | UniProt (P25337) Pfam (PF01125) | Saccharomyces cerevisiae | Bud site selection protein 31,Complexed with CEF1 protein 14 |
| 16 | P (M) | Pre-mRNA-splicing factor CWC2 | polymer | 339 | 38486.6 | 1 | UniProt (Q12046) Pfam (PF16131) Pfam (PF00076) | Saccharomyces cerevisiae | Complexed with CEF1 protein 2,PRP19-associated complex protein 40,Synthetic lethal with CLF1 protein 3 |
| 17 | Q (N) | Pre-mRNA-splicing factor SLT11 | polymer | 364 | 40988.6 | 1 | UniProt (P38241) Pfam (PF21369) | Saccharomyces cerevisiae | Extracellular mutant protein 2,Synthetic lethality with U2 protein 11 |
| 18 | R (O) | Pre-mRNA-splicing factor CEF1 | polymer | 590 | 67837.8 | 1 | UniProt (Q03654) Pfam (PF00249) Pfam (PF11831) | Saccharomyces cerevisiae | PRP nineteen-associated complex protein 85,PRP19-associated complex protein 85 |
| 19 | S (P) | CWC15 | polymer | 175 | 19970.2 | 1 | UniProt (A0A162GIZ3) | Saccharomyces cerevisiae | |
| 20 | T (R) | Pre-mRNA-splicing factor CWC21 | polymer | 135 | 15793.6 | 1 | UniProt (Q03375) Pfam (PF08312) | Saccharomyces cerevisiae | Complexed with CEF1 protein 21 |
| 21 | U (S) | Pre-mRNA-splicing factor CLF1 | polymer | 687 | 82555.9 | 1 | UniProt (Q12309) Pfam (PF23233) Pfam (PF23240) | Saccharomyces cerevisiae | Crooked neck-like factor 1,PRP19-associated complex protein 77,Synthetic lethal with CDC40 protein 3 |
| 22 | V (T) | Pre-mRNA-splicing factor SYF1 | polymer | 859 | 100344.0 | 1 | UniProt (Q04048) Pfam (PF23233) Pfam (PF23220) Pfam (PF23231) | Saccharomyces cerevisiae | PRP19-associated complex protein 90,Synthetic lethal with CDC40 protein 1 |
| 23 | FA, W (b, k) | Small nuclear ribonucleoprotein-associated protein B | polymer | 196 | 22427.0 | 2 | UniProt (P40018) Pfam (PF01423) | Saccharomyces cerevisiae | snRNP-B,Sm protein B,SmB |
| 24 | IA, X (d, n) | Small nuclear ribonucleoprotein Sm D3 | polymer | 101 | 11240.1 | 2 | UniProt (P43321) Pfam (PF01423) | Saccharomyces cerevisiae | Sm-D3,snRNP core protein D3 |
| 25 | JA, Y (e, p) | Small nuclear ribonucleoprotein E | polymer | 94 | 10385.1 | 2 | UniProt (Q12330) Pfam (PF01423) | Saccharomyces cerevisiae | snRNP-E,Sm protein E,SmE |
| 26 | KA, Z (f, q) | Small nuclear ribonucleoprotein F | polymer | 86 | 9669.9 | 2 | UniProt (P54999) Pfam (PF01423) | Saccharomyces cerevisiae | snRNP-F,Sm protein F,SmF |
| 27 | AA, LA (g, r) | Small nuclear ribonucleoprotein G | polymer | 77 | 8490.8 | 2 | UniProt (P40204) Pfam (PF01423) | Saccharomyces cerevisiae | snRNP-G,Sm protein G,SmG |
| 28 | BA, GA (h, l) | Small nuclear ribonucleoprotein Sm D1 | polymer | 146 | 16296.8 | 2 | UniProt (Q02260) Pfam (PF01423) | Saccharomyces cerevisiae | Sm-D1,snRNP core protein D1 |
| 29 | CA, HA (j, m) | Small nuclear ribonucleoprotein Sm D2 | polymer | 110 | 12876.1 | 2 | UniProt (Q06217) Pfam (PF01423) | Saccharomyces cerevisiae | Sm-D2,snRNP core protein D2 |
| 30 | DA (W) | U2 small nuclear ribonucleoprotein A' | polymer | 238 | 27232.3 | 1 | UniProt (Q08963) Pfam (PF14580) | Saccharomyces cerevisiae | U2 snRNP A',Looks exceptionally like U2A protein 1 |
| 31 | EA (Y) | U2 small nuclear ribonucleoprotein B'' | polymer | 111 | 12850.9 | 1 | UniProt (P40567) Pfam (PF13893) | Saccharomyces cerevisiae | U2 snRNP B'' |
| 32 | MA (Q) | Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 | polymer | 1071 | 121815.2 | 1 | UniProt (P15938) Pfam (PF00270) Pfam (PF00271) Pfam (PF04408) Pfam (PF21010) Pfam (PF07717) | Saccharomyces cerevisiae | |
| 33 | NA, OA, PA, QA (t, u, v, w) | Pre-mRNA-processing factor 19 | polymer | 503 | 56629.8 | 4 | UniProt (P32523) Pfam (PF08606) | Saccharomyces cerevisiae | |
| 34 | RA (s) | Pre-mRNA-splicing factor SNT309 | polymer | 175 | 20741.5 | 1 | UniProt (Q06091) | Saccharomyces cerevisiae | PRP19-associated complex protein 25,Synergistic to PRP19 mutation protein 309 |
| 35 | SA (x) | unknown | polymer | 188 | 16017.7 | 1 | Saccharomyces cerevisiae | ||
| 36 | TA, UA (E, V) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) PubChem (888) | |||
| 37 | AB, BB, CB, VA, XA... (M, N, D, L) | ZINC ION | non-polymer | 65.4 | 7 | Chemie (ZN) | |||
| 38 | WA (C) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 45 |
| Total formula weight | 2375092.3 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 1029.7 | |
| All* | Total formula weight | 2376122.0 |
