5LJ3
Structure of the core of the yeast spliceosome immediately after branching
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (U) | U5 snRNA (small nuclear RNA) | polymer | 179 | 57444.9 | 1 | GenBank (1039022920) | Saccharomyces cerevisiae | |
| 2 | B (E) | Exon 1 (5' exon) of UBC4 pre-mRNA | polymer | 16 | 5170.2 | 1 | Saccharomyces cerevisiae | ||
| 3 | C (I) | Intron of UBC4 pre-mRNA | polymer | 76 | 24109.1 | 1 | GenBank (4718) | Saccharomyces cerevisiae | |
| 4 | D (Z) | U2 snRNA (small nuclear RNA) | polymer | 1175 | 376267.4 | 1 | GenBank (536627) | Saccharomyces cerevisiae | |
| 5 | E (V) | U6 snRNA (small nuclear RNA) | polymer | 112 | 35883.2 | 1 | GenBank (1039022925) | Saccharomyces cerevisiae | |
| 6 | F (A) | Pre-mRNA-splicing factor 8 | polymer | 2413 | 279867.5 | 1 | UniProt (P33334) Pfam (PF08082) Pfam (PF08083) Pfam (PF10598) Pfam (PF10597) Pfam (PF10596) Pfam (PF12134) Pfam (PF08084) | Saccharomyces cerevisiae | |
| 7 | G (D) | Protein CWC16 | polymer | 278 | 32371.1 | 1 | UniProt (P28320) Pfam (PF04502) | Saccharomyces cerevisiae | Yju2 |
| 8 | H (F) | Pre-mRNA-splicing factor CWC25 | polymer | 179 | 20412.5 | 1 | UniProt (P53854) Pfam (PF10197) Pfam (PF12542) | Saccharomyces cerevisiae | Complexed with CEF1 protein 25 |
| 9 | I (C) | Pre-mRNA-splicing factor SNU114 | polymer | 1008 | 114174.0 | 1 | UniProt (P36048) Pfam (PF03764) Pfam (PF00679) Pfam (PF16004) Pfam (PF00009) | Saccharomyces cerevisiae | 114 kDa U5 small nuclear ribonucleoprotein component,Growth inhibitory protein 10 |
| 10 | J (G) | ISY1 | polymer | 235 | 28120.9 | 1 | UniProt (A0A165UGV0) Pfam (PF06246) UniProt (by SIFTS) (P21374) | Saccharomyces cerevisiae | |
| 11 | K (H) | CWC22 | polymer | 591 | 69119.0 | 1 | UniProt (A0A162ERE7) | Saccharomyces cerevisiae | |
| 12 | L (J) | PRP46 | polymer | 451 | 50801.3 | 1 | UniProt (A0A162HKW4) Pfam (PF00400) UniProt (by SIFTS) (Q12417) | Saccharomyces cerevisiae | |
| 13 | M (K) | Pre-mRNA-processing protein 45 | polymer | 379 | 42548.7 | 1 | UniProt (P28004) Pfam (PF02731) | Saccharomyces cerevisiae | |
| 14 | N (L) | Pre-mRNA-splicing factor BUD31 | polymer | 157 | 18484.5 | 1 | UniProt (P25337) Pfam (PF01125) | Saccharomyces cerevisiae | Bud site selection protein 31,Complexed with CEF1 protein 14 |
| 15 | O (M) | CWC2 | polymer | 339 | 38458.5 | 1 | UniProt (A0A162HWE4) Pfam (PF16131) Pfam (PF00076) UniProt (by SIFTS) (Q12046) | Saccharomyces cerevisiae | |
| 16 | P (N) | Pre-mRNA-splicing factor SLT11 | polymer | 364 | 40988.6 | 1 | UniProt (P38241) Pfam (PF21369) | Saccharomyces cerevisiae | Extracellular mutant protein 2,Synthetic lethality with U2 protein 11, Ecm2 |
| 17 | Q (O) | CEF1 | polymer | 590 | 67819.8 | 1 | UniProt (A0A165TLN2) Pfam (PF00249) Pfam (PF11831) UniProt (by SIFTS) (Q03654) | Saccharomyces cerevisiae | |
| 18 | R (P) | CWC15 | polymer | 175 | 19970.2 | 1 | UniProt (A0A162GIZ3) Pfam (PF04889) UniProt (by SIFTS) (Q03772) | Saccharomyces cerevisiae | |
| 19 | S (R) | Pre-mRNA-splicing factor CWC21 | polymer | 135 | 15793.6 | 1 | UniProt (Q03375) Pfam (PF08312) | Saccharomyces cerevisiae | Complexed with CEF1 protein 21 |
| 20 | T (S) | CLF1 | polymer | 687 | 82532.9 | 1 | UniProt (A0A165TU20) Pfam (PF23233) Pfam (PF23240) UniProt (by SIFTS) (Q12309) | Saccharomyces cerevisiae | |
| 21 | U (T) | SYF1 | polymer | 859 | 100356.1 | 1 | UniProt (A0A165VVY6) Pfam (PF23233) Pfam (PF23220) Pfam (PF23231) UniProt (by SIFTS) (Q04048) | Saccharomyces cerevisiae (Baker's yeast) | |
| 22 | EA, V (b, k) | Small nuclear ribonucleoprotein-associated protein B | polymer | 196 | 22427.0 | 2 | UniProt (P40018) Pfam (PF01423) | Saccharomyces cerevisiae (Baker's yeast) | snRNP-B,Sm protein B,SmB |
| 23 | HA, W (d, n) | Small nuclear ribonucleoprotein Sm D3 | polymer | 101 | 11240.1 | 2 | UniProt (P43321) Pfam (PF01423) | Saccharomyces cerevisiae (Baker's yeast) | Sm-D3,snRNP core protein D3 |
| 24 | IA, X (e, p) | Small nuclear ribonucleoprotein E | polymer | 94 | 10385.1 | 2 | UniProt (Q12330) Pfam (PF01423) | Saccharomyces cerevisiae (Baker's yeast) | snRNP-E,Sm protein E,SmE |
| 25 | JA, Y (f, q) | Small nuclear ribonucleoprotein F | polymer | 86 | 9669.9 | 2 | UniProt (P54999) Pfam (PF01423) | Saccharomyces cerevisiae (Baker's yeast) | snRNP-F,Sm protein F,SmF |
| 26 | KA, Z (g, r) | Small nuclear ribonucleoprotein G | polymer | 77 | 8490.8 | 2 | UniProt (P40204) Pfam (PF01423) | Saccharomyces cerevisiae (Baker's yeast) | snRNP-G,Sm protein G,SmG |
| 27 | AA, FA (h, l) | Small nuclear ribonucleoprotein Sm D1 | polymer | 146 | 16296.8 | 2 | UniProt (Q02260) Pfam (PF01423) | Saccharomyces cerevisiae (Baker's yeast) | Sm-D1,snRNP core protein D1 |
| 28 | BA, GA (j, m) | Small nuclear ribonucleoprotein Sm D2 | polymer | 110 | 12876.1 | 2 | UniProt (Q06217) Pfam (PF01423) | Saccharomyces cerevisiae (Baker's yeast) | Sm-D2,snRNP core protein D2 |
| 29 | CA (W) | U2 small nuclear ribonucleoprotein A' | polymer | 238 | 27232.3 | 1 | UniProt (Q08963) Pfam (PF14580) | Saccharomyces cerevisiae (Baker's yeast) | U2 snRNP A',Looks exceptionally like U2A protein 1 |
| 30 | DA (Y) | U2 small nuclear ribonucleoprotein B'' | polymer | 111 | 12850.9 | 1 | UniProt (P40567) Pfam (PF13893) | Saccharomyces cerevisiae (Baker's yeast) | U2 snRNP B'' |
| 31 | LA (x) | unknown | polymer | 132 | 11251.9 | 1 | Saccharomyces cerevisiae (Baker's yeast) | ||
| 32 | MA, NA (E, V) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) PubChem (888) | |||
| 33 | OA, QA, RA, SA, TA... (D, L, M, N) | ZINC ION | non-polymer | 65.4 | 7 | Chemie (ZN) | |||
| 34 | PA (C) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 38 |
| Total formula weight | 1754800.6 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 1029.7 | |
| All* | Total formula weight | 1755830.3 |
