5LFA
Crystal structure of iron-sulfur cluster containing bacterial (6-4) photolyase PhrB - Y424F mutant with impaired DNA repair activity
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | (6-4) photolyase | polymer | 518 | 59207.9 | 1 | UniProt (A9CH39) Pfam (PF04244) | Agrobacterium fabrum (strain C58 / ATCC 33970) | (6-4)DNA photolyase,DNA photolyase PhrB,Photoreactivating enzyme PhrB |
| 2 | B (A) | FLAVIN-ADENINE DINUCLEOTIDE | non-polymer | 785.5 | 1 | Chemie (FAD) | |||
| 3 | C (A) | 1-deoxy-1-(6,7-dimethyl-2,4-dioxo-3,4-dihydropteridin-8(2H)-yl)-D-ribitol | non-polymer | 326.3 | 1 | Chemie (DLZ) | |||
| 4 | D (A) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 1 | Chemie (SF4) | |||
| 5 | E, F, G (A) | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
| 6 | H (A) | water | water | 18.0 | 111 | Chemie (HOH) |
Sequence modifications
A: 1 - 507 (UniProt: A9CH39)
| PDB | External Database | Details |
|---|---|---|
| Phe 424 | Tyr 424 | engineered mutation |
| Ala 508 | - | expression tag |
| Ala 509 | - | expression tag |
| Ala 510 | - | expression tag |
| Leu 511 | - | expression tag |
| Glu 512 | - | expression tag |
| His 513 | - | expression tag |
| His 514 | - | expression tag |
| His 515 | - | expression tag |
| His 516 | - | expression tag |
| His 517 | - | expression tag |
| His 518 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 59207.9 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 1739.8 | |
| All* | Total formula weight | 60947.6 |
