5LFA

Crystal structure of iron-sulfur cluster containing bacterial (6-4) photolyase PhrB - Y424F mutant with impaired DNA repair activity

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	(6-4) photolyase	polymer	518	59207.9	1	UniProt (A9CH39) Pfam (PF04244) In PDB	Agrobacterium fabrum (strain C58 / ATCC 33970)	(6-4)DNA photolyase,DNA photolyase PhrB,Photoreactivating enzyme PhrB
2	A	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	1	Chemie (FAD)
3	A	1-deoxy-1-(6,7-dimethyl-2,4-dioxo-3,4-dihydropteridin-8(2H)-yl)-D-ribitol	non-polymer		326.3	1	Chemie (DLZ)
4	A	IRON/SULFUR CLUSTER	non-polymer		351.6	1	Chemie (SF4)
5	A	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
6		water	water		18.0	111	Chemie (HOH)

Sequence modifications

A: 1 - 507 (UniProt: A9CH39)

PDB	External Database	Details
Phe 424	Tyr 424	engineered mutation
Ala 508	-	expression tag
Ala 509	-	expression tag
Ala 510	-	expression tag
Leu 511	-	expression tag
Glu 512	-	expression tag
His 513	-	expression tag
His 514	-	expression tag
His 515	-	expression tag
His 516	-	expression tag
His 517	-	expression tag
His 518	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	59207.9
Non-Polymers*	Number of molecules	6
	Total formula weight	1739.8
All*	Total formula weight	60947.6

*Water molecules are not included.