5LB9

Structure of the T175V Etr1p mutant in the monoclinic form P21

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Enoyl-[acyl-carrier-protein] reductase [NADPH, B-specific] 1, mitochondrial	polymer	385	42087.2	2	UniProt (Q8WZM3) Pfam (PF08240) Pfam (PF00107)	Candida tropicalis (Yeast)	Trans-2-enoyl-CoA reductase 1
2	C, O, P, Q, R... (A, B)	GLYCEROL	non-polymer		92.1	6	Chemie (GOL)
3	D, E, F, H, I... (A, B)	SULFATE ION	non-polymer		96.1	13	Chemie (SO4)
4	G (A)	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	non-polymer		743.4	1	Chemie (NAP)
5	N, W, X (A, B)	SODIUM ION	non-polymer		23.0	3	Chemie (NA)
6	AA, Z (B, A)	water	water		18.0	517	Chemie (HOH)

Sequence modifications

A, B: 23 - 386 (UniProt: Q8WZM3)

PDB	External Database	Details
Met 2	-	initiating methionine
Gly 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
His 11	-	expression tag
His 12	-	expression tag
His 13	-	expression tag
Ser 14	-	expression tag
Ser 15	-	expression tag
Gly 16	-	expression tag
His 17	-	expression tag
Ile 18	-	expression tag
Glu 19	-	expression tag
Gly 20	-	expression tag
Arg 21	-	expression tag
His 22	-	expression tag
Val 175	Thr 175	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	84174.3
Non-Polymers*	Number of molecules	23
	Total formula weight	2613.8
All*	Total formula weight	86788.1

*Water molecules are not included.