5L9Z
Crystal structure of human heparanase nucleophile mutant (E343Q), in complex with unreacted glucuronic acid configured aziridine probe JJB355
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Heparanase | polymer | 389 | 43732.3 | 1 | UniProt (Q9Y251) Pfam (PF03662) | Homo sapiens (Human) | Endo-glucoronidase,Heparanase-1,Hpa1 |
| 2 | B (B) | Heparanase | polymer | 77 | 8542.8 | 1 | UniProt (Q9Y251) | Homo sapiens (Human) | Endo-glucoronidase,Heparanase-1,Hpa1 |
| 3 | C, D, E, F (A) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 4 | Chemie (NAG) | |||
| 4 | G, H, I, J, K... (A, B) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 7 | Chemie (EDO) | |||
| 5 | M, N, O (A) | CHLORIDE ION | non-polymer | 35.5 | 3 | Chemie (CL) | |||
| 6 | P (A) | (1~{R},2~{S},3~{R},4~{S},5~{S},6~{R})-7-[8-[(azanylidene-{4}-azanylidene)amino]octyl]-3,4,5-tris(oxidanyl)-7-azabicyclo[4.1.0]heptane-2-carboxylic acid | non-polymer | 342.4 | 1 | Chemie (GUX) | |||
| 7 | R, S (A, B) | water | water | 18.0 | 402 | Chemie (HOH) |
Sequence modifications
A: 158 - 543 (UniProt: Q9Y251)
B: 36 - 109 (UniProt: Q9Y251)
| PDB | External Database | Details |
|---|---|---|
| Asp 155 | - | expression tag |
| Pro 156 | - | expression tag |
| Gly 157 | - | expression tag |
| Arg 307 | Lys 307 | variant |
| Gln 343 | Glu 343 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Asp 33 | - | expression tag |
| Pro 34 | - | expression tag |
| Gly 35 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 52275.1 | |
| Non-Polymers* | Number of molecules | 15 |
| Total formula weight | 1768.1 | |
| All* | Total formula weight | 54043.2 |
