5L7F
Crystal structure of MMP12 mutant K421A in complex with RXP470.1 conjugated with fluorophore Cy5,5 in space group P21.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Macrophage metalloelastase | polymer | 159 | 17557.6 | 2 | UniProt (P39900) Pfam (PF00413) | Homo sapiens (Human) | MME,Macrophage elastase,hME,Matrix metalloproteinase-12,MMP-12 |
| 2 | C, D, N, O (A, B) | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) | |||
| 3 | E, F, G, P, Q... (A, B) | CALCIUM ION | non-polymer | 40.1 | 6 | Chemie (CA) | |||
| 4 | H (A) | BROMIDE ION | non-polymer | 79.9 | 1 | Chemie (BR) | |||
| 5 | I, J, T (A, B) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 3 | Chemie (EDO) | |||
| 6 | K, S (A, B) | N-[(2S)-3-[(S)-(4-bromophenyl)(hydroxy)phosphoryl]-2-{[3-(3'-chlorobiphenyl-4-yl)-1,2-oxazol-5-yl]methyl}propanoyl]-L-alpha-glutamyl-L-alpha-glutamine | non-polymer | 818.0 | 2 | BIRD (PRD_000830) Chemie (R47) | |||
| 7 | L, M (A, B) | CY5.5-PEG2 | non-polymer | 1092.3 | 2 | Chemie (6PJ) | |||
| 8 | U (B) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 9 | V (B) | DIMETHYL SULFOXIDE | non-polymer | 78.1 | 1 | Chemie (DMS) | |||
| 10 | W, X (A, B) | water | water | 18.0 | 364 | Chemie (HOH) |
Sequence modifications
A, B: 106 - 263 (UniProt: P39900)
| PDB | External Database | Details |
|---|---|---|
| Met 105 | - | initiating methionine |
| Asp 171 | Phe 171 | engineered mutation |
| Ala 241 | Lys 241 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 35115.1 | |
| Non-Polymers* | Number of molecules | 20 |
| Total formula weight | 4759.1 | |
| All* | Total formula weight | 39874.2 |
