5L77
A glycoside hydrolase mutant with an unreacted activity based probe bound
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | beta-glucuronidase | polymer | 495 | 52984.9 | 1 | UniProt (C1F2K5) Pfam (PF03662) Pfam (PF16862) | Acidobacterium capsulatum | |
| 2 | B, C, D, E, F (A) | PHOSPHATE ION | non-polymer | 95.0 | 5 | Chemie (PO4) | |||
| 3 | G (A) | (1~{R},2~{S},3~{R},4~{S},5~{S},6~{R})-7-[8-[(azanylidene-{4}-azanylidene)amino]octyl]-3,4,5-tris(oxidanyl)-7-azabicyclo[4.1.0]heptane-2-carboxylic acid | non-polymer | 342.4 | 1 | Chemie (GUX) | |||
| 4 | H (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 5 | I (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 6 | J (A) | water | water | 18.0 | 483 | Chemie (HOH) |
Sequence modifications
A: 1 - 475 (UniProt: C1F2K5)
| PDB | External Database | Details |
|---|---|---|
| Met -19 | - | initiating methionine |
| Gly -18 | - | expression tag |
| Ser -17 | - | expression tag |
| Ser -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| Ser -9 | - | expression tag |
| Ser -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Val -5 | - | expression tag |
| Pro -4 | - | expression tag |
| Arg -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| His 0 | - | expression tag |
| Gln 287 | Glu 287 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 52984.9 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 944.8 | |
| All* | Total formula weight | 53929.7 |
