5L77

A glycoside hydrolase mutant with an unreacted activity based probe bound

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	beta-glucuronidase	polymer	495	52984.9	1	UniProt (C1F2K5) Pfam (PF03662) Pfam (PF16862) In PDB	Acidobacterium capsulatum
2	A	PHOSPHATE ION	non-polymer		95.0	5	Chemie (PO4)
3	A	(1~{R},2~{S},3~{R},4~{S},5~{S},6~{R})-7-[8-[(azanylidene-{4}-azanylidene)amino]octyl]-3,4,5-tris(oxidanyl)-7-azabicyclo[4.1.0]heptane-2-carboxylic acid	non-polymer		342.4	1	Chemie (GUX)
4	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
5	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
6		water	water		18.0	483	Chemie (HOH)

Sequence modifications

A: 1 - 475 (UniProt: C1F2K5)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Gln 287	Glu 287	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	52984.9
Non-Polymers*	Number of molecules	8
	Total formula weight	944.8
All*	Total formula weight	53929.7

*Water molecules are not included.