5KX5

Crystal structure of tubulin-stathmin-TTL-Compound 11 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Tubulin alpha chain	polymer	451	50188.4	2	UniProt (D0VWZ0) Pfam (PF00091) Pfam (PF03953) In PDB	Ovis aries (Sheep)
2	B, D	Tubulin beta chain	polymer	445	49969.8	2	UniProt (D0VWY9) Pfam (PF00091) Pfam (PF03953) In PDB	Ovis aries (Sheep)
3	E	Stathmin-4	polymer	143	16851.1	1	UniProt (P63043) Pfam (PF00836) In PDB	Rattus norvegicus (Rat)	Stathmin-like protein B3,RB3
4	F	TTL protein	polymer	384	44378.5	1	UniProt (E1BQ43) In PDB	Gallus gallus (Chicken)
5	A, C	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)
6	A, B, C, D	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)
7	A, B, C, E	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
8	B, D	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP)
9	B, D	(2~{S},4~{R})-4-[[2-[(1~{R},3~{R})-1-acetyloxy-3-[[(2~{S},3~{S})-2-[[(2~{R})-1,2-dimethylpyrrolidin-2-yl]carbonylamino]-3-methyl-pentanoyl]-methyl-amino]-4-methyl-pentyl]-1,3-thiazol-4-yl]carbonylamino]-5-(4-aminophenyl)-2-methyl-pentanoic acid	non-polymer		743.0	2	Chemie (6YK)
10	F	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)
11		water	water		18.0	121	Chemie (HOH)

Sequence modifications

E: 5 - 145 (UniProt: P63043)

PDB	External Database	Details
Met 3	-	initiating methionine
Ala 4	-	expression tag
Ala 14	Cys 85	conflict
Trp 20	Phe 91	conflict

F: 1 - 378 (UniProt: E1BQ43)

PDB	External Database	Details
His 379	-	expression tag
His 380	-	expression tag
His 381	-	expression tag
His 382	-	expression tag
His 383	-	expression tag
His 384	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	261546.1
Non-Polymers*	Number of molecules	17
	Total formula weight	4152.0
All*	Total formula weight	265698.1

*Water molecules are not included.